Detailed information of TA system TA127015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3889522..3890321
• Replicon: chromosome
• Accession: NZ_CP040805
• Organism: Escherichia fergusonii strain EFCF056

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: D8Z79_RS18975
• Protein ID: WP_125399928.1
• Coordinates: 3889857..3890321 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: D8Z79_RS18970
• Protein ID: WP_046082024.1
• Coordinates: 3889522..3889857 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z79_RS18955	3885362..3886132	-	771	WP_125399692.1	2-dehydro-3-deoxyglucarate aldolase	-
D8Z79_RS18960	3886275..3887255	+	981	WP_139535862.1	IS5-like element ISKpn26 family transposase	-
D8Z79_RS18965	3887802..3889373	+	1572	WP_046082023.1	galactarate dehydratase	-
D8Z79_RS18970	3889522..3889857	+	336	WP_046082024.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
D8Z79_RS18975	3889857..3890321	+	465	WP_125399928.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
D8Z79_RS18980	3890376..3891185	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
D8Z79_RS18985	3891434..3892714	+	1281	WP_125399926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
D8Z79_RS18990	3892737..3893210	+	474	WP_032303829.1	PTS N-acetylgalactosamine transporter subunit IIB	-
D8Z79_RS18995	3893221..3894000	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
D8Z79_RS19000	3893990..3894868	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
D8Z79_RS19005	3894886..3895320	+	435	WP_000948819.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3886275..3887255	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T127015 WP_125399928.1 NZ_CP040805:3889857-3890321 [Escherichia fergusonii]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGASRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T127015 NZ_CP040805:3889857-3890321 [Escherichia fergusonii]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTAGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12309.91 Da
• Isoelectric Point: 4.4128

>AT127015 WP_046082024.1 NZ_CP040805:3889522-3889857 [Escherichia fergusonii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDPTMNAFLRFLDADIQNN
PQKTRPFDIQQGRKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT127015 NZ_CP040805:3889522-3889857 [Escherichia fergusonii]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGGCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCCTACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAGGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA