Detailed information of TA system TA127010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3268182..3268861
• Replicon: chromosome
• Accession: NZ_CP040805
• Organism: Escherichia fergusonii strain EFCF056

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: D8Z79_RS15775
• Protein ID: WP_002431667.1
• Coordinates: 3268559..3268861 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D8Z79_RS15770
• Protein ID: WP_000806440.1
• Coordinates: 3268182..3268523 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z79_RS15760	3263968..3265257	-	1290	WP_000102764.1	APC family permease	-
D8Z79_RS15765	3265564..3268068	+	2505	WP_125400530.1	copper-exporting P-type ATPase CopA	-
D8Z79_RS15770	3268182..3268523	-	342	WP_000806440.1	HigA family addiction module antidote protein	Antitoxin
D8Z79_RS15775	3268559..3268861	-	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D8Z79_RS15780	3268994..3269788	+	795	WP_125400531.1	TraB/GumN family protein	-
D8Z79_RS15785	3269992..3270471	+	480	WP_000186628.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
D8Z79_RS15790	3270495..3271294	+	800	Protein_3065	hypothetical protein	-
D8Z79_RS15795	3271291..3271794	+	504	WP_000667001.1	hypothetical protein	-
D8Z79_RS15800	3271832..3273484	-	1653	WP_125400532.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T127010 WP_002431667.1 NZ_CP040805:c3268861-3268559 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T127010 NZ_CP040805:c3268861-3268559 [Escherichia fergusonii]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCACTCAACGCCACATAGAAA
GATTCCTGCTGAAATCCATACCACATTATCAAGAAAATTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTATTTAGATCCACACAACTACTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13157.08 Da
• Isoelectric Point: 5.7737

>AT127010 WP_000806440.1 NZ_CP040805:c3268523-3268182 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT127010 NZ_CP040805:c3268523-3268182 [Escherichia fergusonii]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAGCCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACAACTG
AAATGGCGTTTCGTCTGGCTAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACACAAGAAGAATTGGGACGGATTGAAACAGTGGCTGACTATTTGGCTCGCCGTGAAGA
GCGTGCAAAGAAGGTTGCGTAA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8