Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1682354..1682856 Replicon chromosome
Accession NZ_CP040805
Organism Escherichia fergusonii strain EFCF056

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag D8Z79_RS08025 Protein ID WP_000767829.1
Coordinates 1682602..1682856 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag D8Z79_RS08020 Protein ID WP_001259255.1
Coordinates 1682354..1682605 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z79_RS08000 1678508..1679525 - 1018 Protein_1545 IS110 family transposase -
D8Z79_RS08005 1679668..1680969 - 1302 WP_125400932.1 histidinol dehydrogenase -
D8Z79_RS08010 1680975..1681874 - 900 WP_021544491.1 ATP phosphoribosyltransferase -
D8Z79_RS08015 1682020..1682070 - 51 WP_001364200.1 his operon leader peptide -
D8Z79_RS08020 1682354..1682605 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
D8Z79_RS08025 1682602..1682856 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
D8Z79_RS08030 1682935..1683759 + 825 WP_000754745.1 SDR family oxidoreductase -
D8Z79_RS08035 1683806..1684735 + 930 WP_125400931.1 LysR family transcriptional regulator -
D8Z79_RS08040 1684949..1685011 + 63 WP_010723108.1 membrane protein YoeI -
D8Z79_RS08045 1685001..1686359 + 1359 WP_000019201.1 putrescine/proton symporter PlaP -
D8Z79_RS08050 1686537..1686971 + 435 WP_038989254.1 IS200/IS605 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1679142..1679525 383
- flank IS/Tn - - 1686537..1686971 434


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T127000 WP_000767829.1 NZ_CP040805:1682602-1682856 [Escherichia fergusonii]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T127000 NZ_CP040805:1682602-1682856 [Escherichia fergusonii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACTCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT127000 WP_001259255.1 NZ_CP040805:1682354-1682605 [Escherichia fergusonii]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT127000 NZ_CP040805:1682354-1682605 [Escherichia fergusonii]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAACGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAAGAGACGGCTTATC
TATTGCGTTCCCCCGCTAATGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References