Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 973904..974389 Replicon chromosome
Accession NZ_CP040780
Organism Lacticaseibacillus rhamnosus strain 1.0320

Toxin (Protein)


Gene name yafQ Uniprot ID C2K0Y1
Locus tag FG342_RS04870 Protein ID WP_005691636.1
Coordinates 973904..974089 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A249DC59
Locus tag FG342_RS04875 Protein ID WP_047676897.1
Coordinates 974117..974389 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FG342_RS04845 968911..969600 + 690 WP_047676891.1 rhomboid family intramembrane serine protease -
FG342_RS04850 970092..970700 - 609 WP_047676893.1 hypothetical protein -
FG342_RS04860 971147..972022 + 876 WP_024305787.1 XRE family transcriptional regulator -
FG342_RS04865 972265..973680 - 1416 WP_047676895.1 DNA polymerase III subunit epsilon -
FG342_RS04870 973904..974089 - 186 WP_005691636.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
FG342_RS04875 974117..974389 - 273 WP_047676897.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
FG342_RS04880 974951..975199 + 249 WP_005691639.1 hypothetical protein -
FG342_RS04885 975442..975735 + 294 WP_005684269.1 metalloregulator ArsR/SmtB family transcription factor -
FG342_RS04890 975817..976980 + 1164 WP_005691640.1 MFS transporter -
FG342_RS04895 977134..977709 + 576 WP_047676900.1 DNA-3-methyladenine glycosylase I -
FG342_RS04900 977831..978049 + 219 WP_047676903.1 hypothetical protein -
FG342_RS04905 978178..979059 - 882 WP_005684263.1 class II fructose-1,6-bisphosphate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(8-57)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-84)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6781.86 Da        Isoelectric Point: 7.4698

>T126957 WP_005691636.1 NZ_CP040780:c974089-973904 [Lacticaseibacillus rhamnosus]
MQRQGHVKLASLHDHALKGAHSGERALRVAPDWLLVYKVDAEALILMLLATGTHRDTLNIE

Download         Length: 186 bp

>T126957 NZ_CP040780:c974089-973904 [Lacticaseibacillus rhamnosus]
TTGCAGCGTCAAGGTCATGTAAAATTAGCTTCTCTACATGACCATGCTTTAAAGGGTGCTCACAGCGGCGAACGAGCATT
GCGTGTTGCTCCTGATTGGCTTCTCGTTTATAAAGTCGATGCTGAAGCGTTAATTTTAATGCTTCTTGCAACAGGCACAC
ACCGCGATACATTGAACATTGAGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9979.58 Da        Isoelectric Point: 9.5939

>AT126957 WP_047676897.1 NZ_CP040780:c974389-974117 [Lacticaseibacillus rhamnosus]
MTKKSRISVRIDTKTKERALHVLNSMGLDISSAINMYLKRIGDTGALPFTPAMSFVDQLQVAEADVKAGRIKSFKTVDAL
MKDLYSDVDD

Download         Length: 273 bp

>AT126957 NZ_CP040780:c974389-974117 [Lacticaseibacillus rhamnosus]
ATGACCAAAAAATCCCGCATCAGCGTTAGGATTGATACCAAAACTAAAGAACGAGCGCTCCATGTTCTTAATAGCATGGG
ATTAGATATTTCCTCGGCTATTAACATGTACCTGAAACGGATTGGTGACACCGGTGCATTGCCATTTACACCTGCAATGT
CATTTGTTGATCAGCTTCAAGTTGCAGAAGCTGATGTTAAAGCGGGGCGAATAAAAAGCTTCAAGACTGTCGATGCTTTG
ATGAAGGATTTATACAGTGATGTTGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X2J6C9

Antitoxin

Source ID Structure
AlphaFold DB A0A249DC59

References