Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 225276..225811 Replicon plasmid pVRE1-1
Accession NZ_CP040741
Organism Enterococcus faecium strain VRE1

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag FGY81_RS15075 Protein ID WP_002314366.1
Coordinates 225276..225548 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag FGY81_RS15080 Protein ID WP_002297422.1
Coordinates 225548..225811 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGY81_RS15040 221222..221548 - 327 Protein_231 2-dehydro-3-deoxyphosphogluconate aldolase -
FGY81_RS15045 221593..222273 - 681 WP_044383156.1 IS6 family transposase -
FGY81_RS15050 222348..222643 - 296 Protein_233 hypothetical protein -
FGY81_RS15055 222903..223451 - 549 WP_002292681.1 hypothetical protein -
FGY81_RS15060 223452..224309 - 858 WP_002292680.1 AAA family ATPase -
FGY81_RS15065 224593..224880 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
FGY81_RS15070 224870..225199 - 330 WP_002292678.1 AbrB family transcriptional regulator -
FGY81_RS15075 225276..225548 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
FGY81_RS15080 225548..225811 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FGY81_RS15085 226045..226953 + 909 Protein_240 IS982-like element ISEfm1 family transposase -
FGY81_RS15090 226956..228131 - 1176 WP_002336205.1 MFS transporter -
FGY81_RS15095 228134..230383 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..238664 238664
- inside IScluster/Tn - - 221593..236412 14819


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T126936 WP_002314366.1 NZ_CP040741:c225548-225276 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T126936 NZ_CP040741:c225548-225276 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT126936 WP_002297422.1 NZ_CP040741:c225811-225548 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT126936 NZ_CP040741:c225811-225548 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References