Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 99253..99980 | Replicon | plasmid p16HN-35_Vir |
| Accession | NZ_CP040712 | ||
| Organism | Klebsiella pneumoniae strain 16HN-35 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | FGL96_RS27535 | Protein ID | WP_011251285.1 |
| Coordinates | 99669..99980 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | FGL96_RS27530 | Protein ID | WP_011251286.1 |
| Coordinates | 99253..99672 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FGL96_RS27500 (94421) | 94421..94891 | - | 471 | WP_048333570.1 | hypothetical protein | - |
| FGL96_RS27505 (95204) | 95204..95839 | - | 636 | WP_223171879.1 | hypothetical protein | - |
| FGL96_RS27510 (96258) | 96258..96878 | + | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| FGL96_RS27515 (96899) | 96899..97687 | + | 789 | WP_040217257.1 | hypothetical protein | - |
| FGL96_RS27520 (97701) | 97701..98066 | + | 366 | WP_048333448.1 | hypothetical protein | - |
| FGL96_RS27525 (98138) | 98138..99106 | - | 969 | WP_011251287.1 | IS5 family transposase | - |
| FGL96_RS27530 (99253) | 99253..99672 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| FGL96_RS27535 (99669) | 99669..99980 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| FGL96_RS27540 (100185) | 100185..100622 | - | 438 | Protein_119 | DDE-type integrase/transposase/recombinase | - |
| FGL96_RS27545 (100757) | 100757..101454 | + | 698 | WP_223175001.1 | IS1-like element IS1A family transposase | - |
| FGL96_RS27550 (101456) | 101456..101905 | + | 450 | WP_011251283.1 | thioredoxin fold domain-containing protein | - |
| FGL96_RS27555 (101922) | 101922..102233 | + | 312 | WP_011251282.1 | hypothetical protein | - |
| FGL96_RS27560 (102247) | 102247..102588 | + | 342 | WP_011251281.1 | hypothetical protein | - |
| FGL96_RS27565 (102648) | 102648..103604 | + | 957 | WP_011251280.1 | DsbA family protein | - |
| FGL96_RS27570 (104029) | 104029..104469 | - | 441 | WP_011251275.1 | hypothetical protein | - |
| FGL96_RS27575 (104475) | 104475..104969 | - | 495 | WP_011251274.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | dfrA17 / aadA5 / qacE / sul1 / mph(A) | iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA | 1..270854 | 270854 | |
| - | inside | IScluster/Tn | - | - | 89132..106889 | 17757 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T126889 WP_011251285.1 NZ_CP040712:c99980-99669 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T126889 NZ_CP040712:c99980-99669 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT126889 WP_011251286.1 NZ_CP040712:c99672-99253 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT126889 NZ_CP040712:c99672-99253 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|