Detailed information of TA system TA126885

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36176..36602
• Replicon: plasmid p16HN-35_Vir
• Accession: NZ_CP040712
• Organism: Klebsiella pneumoniae strain 16HN-35

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: FGL96_RS27175
• Protein ID: WP_001372321.1
• Coordinates: 36176..36301 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	36378..36602 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGL96_RS27135 (31214)	31214..31441	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
FGL96_RS27140 (31535)	31535..32221	-	687	WP_000332487.1	PAS domain-containing protein	-
FGL96_RS27145 (32415)	32415..32798	-	384	WP_001151564.1	conjugal transfer relaxosome DNA-binding protein TraM	-
FGL96_RS27150 (33093)	33093..33731	+	639	WP_281440555.1	transglycosylase SLT domain-containing protein	-
FGL96_RS27155 (34028)	34028..34849	-	822	WP_001234469.1	DUF932 domain-containing protein	-
FGL96_RS27160 (34970)	34970..35257	-	288	WP_000107537.1	hypothetical protein	-
FGL96_RS27165 (35555)	35555..35728	+	174	Protein_44	hypothetical protein	-
FGL96_RS27170 (35726)	35726..35956	-	231	WP_071587244.1	hypothetical protein	-
FGL96_RS27175 (36176)	36176..36301	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
FGL96_RS27180 (36243)	36243..36392	-	150	Protein_47	plasmid maintenance protein Mok	-
- (36378)	36378..36602	-	225	NuclAT_0	-	Antitoxin
- (36378)	36378..36602	-	225	NuclAT_0	-	Antitoxin
- (36378)	36378..36602	-	225	NuclAT_0	-	Antitoxin
- (36378)	36378..36602	-	225	NuclAT_0	-	Antitoxin
FGL96_RS27185 (36571)	36571..37333	-	763	Protein_48	plasmid SOS inhibition protein A	-
FGL96_RS27190 (37330)	37330..37764	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
FGL96_RS27195 (37819)	37819..39777	-	1959	WP_032152921.1	ParB/RepB/Spo0J family partition protein	-
FGL96_RS27200 (39836)	39836..40069	-	234	WP_000006018.1	DUF905 domain-containing protein	-
FGL96_RS27205 (40125)	40125..40652	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
FGL96_RS27210 (41122)	41122..41370	-	249	WP_071606928.1	hypothetical protein	-
FGL96_RS27215 (41292)	41292..41522	-	231	WP_001333093.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A)	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA	1..270854	270854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T126885 WP_001372321.1 NZ_CP040712:c36301-36176 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T126885 NZ_CP040712:c36301-36176 [Klebsiella pneumoniae]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT126885 NZ_CP040712:c36602-36378 [Klebsiella pneumoniae]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin