Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2188522..2189390 Replicon chromosome
Accession NZ_CP040693
Organism Mycobacterium tuberculosis complex sp. N0145

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag FF953_RS10190 Protein ID WP_010886136.1
Coordinates 2188522..2188899 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag FF953_RS10195 Protein ID WP_003409886.1
Coordinates 2188941..2189390 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF953_RS10140 (FF953_10505) 2184311..2184763 - 453 WP_003899095.1 lipoprotein -
FF953_RS10145 (FF953_10510) 2184827..2185228 + 402 WP_003409869.1 hypothetical protein -
FF953_RS10150 (FF953_10515) 2185221..2185403 - 183 WP_003409870.1 hypothetical protein -
FF953_RS10155 (FF953_10520) 2185517..2185867 - 351 WP_003409871.1 hypothetical protein -
FF953_RS10160 (FF953_10525) 2185878..2186780 - 903 WP_003899097.1 hypothetical protein -
FF953_RS10165 (FF953_10530) 2186801..2186992 - 192 WP_003409876.1 hypothetical protein -
FF953_RS10170 (FF953_10535) 2186993..2187289 - 297 WP_031715189.1 hypothetical protein -
FF953_RS10175 (FF953_10540) 2187529..2187744 + 216 WP_003409878.1 antitoxin -
FF953_RS10180 (FF953_10545) 2187741..2188052 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
FF953_RS10185 2188026..2188547 - 522 WP_003904745.1 hypothetical protein -
FF953_RS10190 (FF953_10555) 2188522..2188899 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
FF953_RS10195 (FF953_10560) 2188941..2189390 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
FF953_RS10200 (FF953_10565) 2189387..2189932 + 546 WP_003409891.1 SecB-like chaperone -
FF953_RS10205 (FF953_10570) 2189821..2190435 - 615 WP_003901296.1 hypothetical protein -
FF953_RS10210 (FF953_10575) 2190484..2190780 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
FF953_RS10215 (FF953_10580) 2190777..2191028 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
FF953_RS10220 (FF953_10585) 2191015..2191509 + 495 WP_003899099.1 hypothetical protein -
FF953_RS10225 (FF953_10590) 2191669..2192076 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
FF953_RS10230 (FF953_10595) 2192080..2192352 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FF953_RS10235 (FF953_10600) 2192385..2193605 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T126830 WP_010886136.1 NZ_CP040693:2188522-2188899 [Mycobacterium tuberculosis complex sp. N0145]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T126830 NZ_CP040693:2188522-2188899 [Mycobacterium tuberculosis complex sp. N0145]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT126830 WP_003409886.1 NZ_CP040693:2188941-2189390 [Mycobacterium tuberculosis complex sp. N0145]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT126830 NZ_CP040693:2188941-2189390 [Mycobacterium tuberculosis complex sp. N0145]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCATCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References