Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2200219..2201087 Replicon chromosome
Accession NZ_CP040692
Organism Mycobacterium tuberculosis complex sp. N0157

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag FF956_RS10350 Protein ID WP_010886136.1
Coordinates 2200710..2201087 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag FF956_RS10345 Protein ID WP_003409886.1
Coordinates 2200219..2200668 (-) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF956_RS10305 (FF956_10755) 2196004..2197224 + 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -
FF956_RS10310 (FF956_10760) 2197257..2197529 + 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FF956_RS10315 (FF956_10765) 2197533..2197940 + 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
FF956_RS10320 (FF956_10770) 2198100..2198594 - 495 WP_003899099.1 hypothetical protein -
FF956_RS10325 (FF956_10775) 2198581..2198832 + 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
FF956_RS10330 (FF956_10780) 2198829..2199125 + 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
FF956_RS10335 (FF956_10785) 2199174..2199788 + 615 WP_003901296.1 hypothetical protein -
FF956_RS10340 (FF956_10790) 2199677..2200222 - 546 WP_003409891.1 SecB-like chaperone -
FF956_RS10345 (FF956_10795) 2200219..2200668 - 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
FF956_RS10350 (FF956_10800) 2200710..2201087 - 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
FF956_RS10355 2201062..2201583 + 522 WP_003903694.1 hypothetical protein -
FF956_RS10360 (FF956_10810) 2201557..2201868 - 312 WP_003903693.1 type II toxin-antitoxin system VapC family toxin -
FF956_RS10365 (FF956_10815) 2201865..2202080 - 216 WP_003409878.1 antitoxin -
FF956_RS10370 (FF956_10820) 2202320..2202616 + 297 WP_003409877.1 hypothetical protein -
FF956_RS10375 (FF956_10825) 2202617..2202808 + 192 WP_003409876.1 hypothetical protein -
FF956_RS10380 (FF956_10830) 2202829..2203731 + 903 WP_003409874.1 hypothetical protein -
FF956_RS10385 (FF956_10835) 2203742..2204092 + 351 WP_003409871.1 hypothetical protein -
FF956_RS10390 (FF956_10840) 2204206..2204388 + 183 WP_003409870.1 hypothetical protein -
FF956_RS10395 (FF956_10845) 2204381..2204782 - 402 WP_003409869.1 hypothetical protein -
FF956_RS10400 (FF956_10850) 2204846..2205298 + 453 WP_003899095.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T126766 WP_010886136.1 NZ_CP040692:c2201087-2200710 [Mycobacterium tuberculosis complex sp. N0157]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T126766 NZ_CP040692:c2201087-2200710 [Mycobacterium tuberculosis complex sp. N0157]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT126766 WP_003409886.1 NZ_CP040692:c2200668-2200219 [Mycobacterium tuberculosis complex sp. N0157]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT126766 NZ_CP040692:c2200668-2200219 [Mycobacterium tuberculosis complex sp. N0157]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References