Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2188407..2189275 Replicon chromosome
Accession NZ_CP040691
Organism Mycobacterium tuberculosis complex sp. N0155

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag FF955_RS10195 Protein ID WP_010886136.1
Coordinates 2188407..2188784 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag FF955_RS10200 Protein ID WP_003409886.1
Coordinates 2188826..2189275 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF955_RS10145 (FF955_10500) 2184196..2184648 - 453 WP_003899095.1 lipoprotein -
FF955_RS10150 (FF955_10505) 2184712..2185113 + 402 WP_003409869.1 hypothetical protein -
FF955_RS10155 (FF955_10510) 2185106..2185288 - 183 WP_003409870.1 hypothetical protein -
FF955_RS10160 (FF955_10515) 2185402..2185752 - 351 WP_003409871.1 hypothetical protein -
FF955_RS10165 (FF955_10520) 2185763..2186665 - 903 WP_003899097.1 hypothetical protein -
FF955_RS10170 (FF955_10525) 2186686..2186877 - 192 WP_003409876.1 hypothetical protein -
FF955_RS10175 (FF955_10530) 2186878..2187174 - 297 WP_003409877.1 hypothetical protein -
FF955_RS10180 (FF955_10535) 2187414..2187629 + 216 WP_003409878.1 antitoxin -
FF955_RS10185 (FF955_10540) 2187626..2187937 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
FF955_RS10190 2187911..2188432 - 522 WP_003904745.1 hypothetical protein -
FF955_RS10195 (FF955_10550) 2188407..2188784 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
FF955_RS10200 (FF955_10555) 2188826..2189275 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
FF955_RS10205 (FF955_10560) 2189272..2189817 + 546 WP_003409891.1 SecB-like chaperone -
FF955_RS10210 (FF955_10565) 2189706..2190320 - 615 WP_003901296.1 hypothetical protein -
FF955_RS10215 (FF955_10570) 2190369..2190665 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
FF955_RS10220 (FF955_10575) 2190662..2190913 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
FF955_RS10225 (FF955_10580) 2190900..2191394 + 495 WP_003899099.1 hypothetical protein -
FF955_RS10230 (FF955_10585) 2191554..2191961 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
FF955_RS10235 (FF955_10590) 2191965..2192237 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FF955_RS10240 (FF955_10595) 2192270..2193490 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T126709 WP_010886136.1 NZ_CP040691:2188407-2188784 [Mycobacterium tuberculosis complex sp. N0155]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T126709 NZ_CP040691:2188407-2188784 [Mycobacterium tuberculosis complex sp. N0155]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT126709 WP_003409886.1 NZ_CP040691:2188826-2189275 [Mycobacterium tuberculosis complex sp. N0155]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT126709 NZ_CP040691:2188826-2189275 [Mycobacterium tuberculosis complex sp. N0155]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCATCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References