Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3541185..3541855 Replicon chromosome
Accession NZ_CP040690
Organism Mycobacterium tuberculosis complex sp. N0153

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag FF954_RS16725 Protein ID WP_003899954.1
Coordinates 3541185..3541529 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag FF954_RS16730 Protein ID WP_003899955.1
Coordinates 3541526..3541855 (+) Length 110 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF954_RS16690 (FF954_17275) 3536258..3537118 + 861 WP_003416628.1 alpha/beta hydrolase -
FF954_RS16695 (FF954_17280) 3537093..3537608 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
FF954_RS16700 (FF954_17285) 3537624..3537835 + 212 Protein_3297 (R)-hydratase -
FF954_RS16705 (FF954_17290) 3537848..3538141 + 294 WP_003416635.1 hypothetical protein -
FF954_RS16710 (FF954_17295) 3538429..3539718 + 1290 WP_003416640.1 ATP-binding protein -
FF954_RS16715 (FF954_17300) 3540065..3540499 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
FF954_RS16720 (FF954_17305) 3540502..3540954 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FF954_RS16725 (FF954_17310) 3541185..3541529 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FF954_RS16730 (FF954_17315) 3541526..3541855 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
FF954_RS16735 (FF954_17320) 3542393..3542740 + 348 WP_003899956.1 DUF2384 domain-containing protein -
FF954_RS16740 (FF954_17325) 3542737..3543357 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
FF954_RS16745 (FF954_17330) 3543517..3544782 - 1266 WP_003909837.1 hypothetical protein -
FF954_RS16750 (FF954_17335) 3544950..3545159 + 210 WP_003416778.1 hypothetical protein -
FF954_RS16755 (FF954_17345) 3545406..3546440 - 1035 WP_003905011.1 IS30 family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-103)

Antitoxin

(38-91)


Sequences


Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T126673 WP_003899954.1 NZ_CP040690:3541185-3541529 [Mycobacterium tuberculosis complex sp. N0153]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T126673 NZ_CP040690:3541185-3541529 [Mycobacterium tuberculosis complex sp. N0153]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT126673 WP_003899955.1 NZ_CP040690:3541526-3541855 [Mycobacterium tuberculosis complex sp. N0153]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT126673 NZ_CP040690:3541526-3541855 [Mycobacterium tuberculosis complex sp. N0153]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2


Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References