Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2201778..2202646 Replicon chromosome
Accession NZ_CP040690
Organism Mycobacterium tuberculosis complex sp. N0153

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag FF954_RS10235 Protein ID WP_010886136.1
Coordinates 2201778..2202155 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag FF954_RS10240 Protein ID WP_003409886.1
Coordinates 2202197..2202646 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF954_RS10185 (FF954_10540) 2197567..2198019 - 453 WP_003899095.1 lipoprotein -
FF954_RS10190 (FF954_10545) 2198083..2198484 + 402 WP_003409869.1 hypothetical protein -
FF954_RS10195 (FF954_10550) 2198477..2198659 - 183 WP_003409870.1 hypothetical protein -
FF954_RS10200 (FF954_10555) 2198773..2199123 - 351 WP_003409871.1 hypothetical protein -
FF954_RS10205 (FF954_10560) 2199134..2200036 - 903 WP_003409874.1 hypothetical protein -
FF954_RS10210 (FF954_10565) 2200057..2200248 - 192 WP_003409876.1 hypothetical protein -
FF954_RS10215 (FF954_10570) 2200249..2200545 - 297 WP_003409877.1 hypothetical protein -
FF954_RS10220 (FF954_10575) 2200785..2201000 + 216 WP_003409878.1 antitoxin -
FF954_RS10225 (FF954_10580) 2200997..2201308 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
FF954_RS10230 2201282..2201803 - 522 WP_003904745.1 hypothetical protein -
FF954_RS10235 (FF954_10590) 2201778..2202155 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
FF954_RS10240 (FF954_10595) 2202197..2202646 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
FF954_RS10245 (FF954_10600) 2202643..2203188 + 546 WP_031663732.1 hypothetical protein -
FF954_RS10250 (FF954_10605) 2203077..2203691 - 615 WP_003901296.1 hypothetical protein -
FF954_RS10255 (FF954_10610) 2203740..2204036 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
FF954_RS10260 (FF954_10615) 2204033..2204284 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
FF954_RS10265 (FF954_10620) 2204271..2204765 + 495 WP_003899099.1 hypothetical protein -
FF954_RS10270 (FF954_10625) 2204925..2205332 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
FF954_RS10275 (FF954_10630) 2205336..2205608 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FF954_RS10280 (FF954_10635) 2205641..2206861 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T126650 WP_010886136.1 NZ_CP040690:2201778-2202155 [Mycobacterium tuberculosis complex sp. N0153]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T126650 NZ_CP040690:2201778-2202155 [Mycobacterium tuberculosis complex sp. N0153]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT126650 WP_003409886.1 NZ_CP040690:2202197-2202646 [Mycobacterium tuberculosis complex sp. N0153]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT126650 NZ_CP040690:2202197-2202646 [Mycobacterium tuberculosis complex sp. N0153]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References