Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2214462..2215330 Replicon chromosome
Accession NZ_CP040689
Organism Mycobacterium tuberculosis complex sp. N0072

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag FF952_RS10320 Protein ID WP_010886136.1
Coordinates 2214462..2214839 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag FF952_RS10325 Protein ID WP_003409886.1
Coordinates 2214881..2215330 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF952_RS10270 (FF952_10615) 2210251..2210703 - 453 WP_003899095.1 lipoprotein -
FF952_RS10275 (FF952_10620) 2210767..2211168 + 402 WP_003409869.1 hypothetical protein -
FF952_RS10280 (FF952_10625) 2211161..2211343 - 183 WP_003409870.1 hypothetical protein -
FF952_RS10285 (FF952_10630) 2211457..2211807 - 351 WP_003409871.1 hypothetical protein -
FF952_RS10290 (FF952_10635) 2211818..2212720 - 903 WP_003409874.1 hypothetical protein -
FF952_RS10295 (FF952_10640) 2212741..2212932 - 192 WP_003409876.1 hypothetical protein -
FF952_RS10300 (FF952_10645) 2212933..2213229 - 297 WP_003409877.1 hypothetical protein -
FF952_RS10305 (FF952_10650) 2213469..2213684 + 216 WP_003409878.1 antitoxin -
FF952_RS10310 (FF952_10655) 2213681..2213992 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
FF952_RS10315 2213966..2214487 - 522 WP_003904745.1 hypothetical protein -
FF952_RS10320 (FF952_10665) 2214462..2214839 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
FF952_RS10325 (FF952_10670) 2214881..2215330 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
FF952_RS10330 (FF952_10675) 2215327..2215872 + 546 WP_003409891.1 SecB-like chaperone -
FF952_RS10335 (FF952_10680) 2215761..2216375 - 615 WP_003901296.1 hypothetical protein -
FF952_RS10340 (FF952_10685) 2216424..2216720 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
FF952_RS10345 (FF952_10690) 2216717..2216968 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
FF952_RS10350 (FF952_10695) 2216955..2217449 + 495 WP_003899099.1 hypothetical protein -
FF952_RS10355 (FF952_10700) 2217609..2218016 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
FF952_RS10360 (FF952_10705) 2218020..2218292 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FF952_RS10365 (FF952_10710) 2218325..2219545 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T126590 WP_010886136.1 NZ_CP040689:2214462-2214839 [Mycobacterium tuberculosis complex sp. N0072]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T126590 NZ_CP040689:2214462-2214839 [Mycobacterium tuberculosis complex sp. N0072]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT126590 WP_003409886.1 NZ_CP040689:2214881-2215330 [Mycobacterium tuberculosis complex sp. N0072]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT126590 NZ_CP040689:2214881-2215330 [Mycobacterium tuberculosis complex sp. N0072]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References