Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3537973..3538643 Replicon chromosome
Accession NZ_CP040688
Organism Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag FF951_RS16785 Protein ID WP_003899954.1
Coordinates 3537973..3538317 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag FF951_RS16790 Protein ID WP_003899955.1
Coordinates 3538314..3538643 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF951_RS16755 (FF951_17335) 3533054..3533265 + 212 Protein_3308 (R)-hydratase -
FF951_RS16760 (FF951_17340) 3533278..3533571 + 294 WP_003416635.1 hypothetical protein -
FF951_RS16765 (FF951_17345) 3533859..3535148 + 1290 WP_003416640.1 ATP-binding protein -
FF951_RS16775 (FF951_17355) 3536853..3537287 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
FF951_RS16780 (FF951_17360) 3537290..3537742 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FF951_RS16785 (FF951_17365) 3537973..3538317 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FF951_RS16790 (FF951_17370) 3538314..3538643 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
FF951_RS16795 (FF951_17375) 3539181..3539528 + 348 WP_003899956.1 DUF2384 domain-containing protein -
FF951_RS16800 (FF951_17380) 3539525..3540145 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
FF951_RS16805 (FF951_17385) 3540305..3541570 - 1266 WP_003902423.1 hypothetical protein -
FF951_RS16810 (FF951_17390) 3541738..3541947 + 210 WP_003416778.1 hypothetical protein -
FF951_RS16815 (FF951_17400) 3542194..3543228 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T126553 WP_003899954.1 NZ_CP040688:3537973-3538317 [Mycobacterium tuberculosis complex sp. N0052]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T126553 NZ_CP040688:3537973-3538317 [Mycobacterium tuberculosis complex sp. N0052]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT126553 WP_003899955.1 NZ_CP040688:3538314-3538643 [Mycobacterium tuberculosis complex sp. N0052]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT126553 NZ_CP040688:3538314-3538643 [Mycobacterium tuberculosis complex sp. N0052]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References