Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2252562..2253481 Replicon chromosome
Accession NZ_CP040688
Organism Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag FF951_RS10600 Protein ID WP_003900449.1
Coordinates 2252876..2253481 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag FF951_RS10595 Protein ID WP_003410124.1
Coordinates 2252562..2252867 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF951_RS10560 (FF951_10920) 2247826..2248026 + 201 Protein_2087 hypothetical protein -
FF951_RS10570 (FF951_10930) 2249382..2249758 + 377 Protein_2089 hypothetical protein -
FF951_RS10575 (FF951_10935) 2249755..2250795 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
FF951_RS10580 (FF951_10940) 2251037..2251756 + 720 WP_003410108.1 DUF433 domain-containing protein -
FF951_RS10585 (FF951_10945) 2251746..2252162 + 417 WP_003410114.1 hypothetical protein -
FF951_RS10590 (FF951_10950) 2252178..2252477 - 300 WP_003410120.1 hypothetical protein -
FF951_RS10595 (FF951_10955) 2252562..2252867 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
FF951_RS10600 (FF951_10960) 2252876..2253481 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FF951_RS10605 (FF951_10965) 2253506..2253865 - 360 WP_003410131.1 hypothetical protein -
FF951_RS10610 (FF951_10970) 2254025..2254657 - 633 WP_233368240.1 hypothetical protein -
FF951_RS10615 (FF951_10975) 2254654..2254890 - 237 WP_003410133.1 hypothetical protein -
FF951_RS10620 (FF951_10980) 2255097..2255993 - 897 WP_003410136.1 hypothetical protein -
FF951_RS10625 (FF951_10985) 2256177..2256587 - 411 WP_286449291.1 hypothetical protein -
FF951_RS10630 (FF951_10990) 2256586..2257847 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T126535 WP_003900449.1 NZ_CP040688:c2253481-2252876 [Mycobacterium tuberculosis complex sp. N0052]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T126535 NZ_CP040688:c2253481-2252876 [Mycobacterium tuberculosis complex sp. N0052]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT126535 WP_003410124.1 NZ_CP040688:c2252867-2252562 [Mycobacterium tuberculosis complex sp. N0052]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT126535 NZ_CP040688:c2252867-2252562 [Mycobacterium tuberculosis complex sp. N0052]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCGTTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References