Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2181623..2182491 Replicon chromosome
Accession NZ_CP040688
Organism Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag FF951_RS10180 Protein ID WP_010886136.1
Coordinates 2181623..2182000 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag FF951_RS10185 Protein ID WP_003409886.1
Coordinates 2182042..2182491 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF951_RS10130 (FF951_10460) 2177412..2177864 - 453 WP_003899095.1 lipoprotein -
FF951_RS10135 (FF951_10465) 2177928..2178329 + 402 WP_003409869.1 hypothetical protein -
FF951_RS10140 (FF951_10470) 2178322..2178504 - 183 WP_019250140.1 hypothetical protein -
FF951_RS10145 (FF951_10475) 2178618..2178968 - 351 WP_003409871.1 hypothetical protein -
FF951_RS10150 (FF951_10480) 2178979..2179881 - 903 WP_003899097.1 hypothetical protein -
FF951_RS10155 (FF951_10485) 2179902..2180093 - 192 WP_003409876.1 hypothetical protein -
FF951_RS10160 (FF951_10490) 2180094..2180390 - 297 WP_003409877.1 hypothetical protein -
FF951_RS10165 (FF951_10495) 2180630..2180845 + 216 WP_003409878.1 antitoxin -
FF951_RS10170 (FF951_10500) 2180842..2181153 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
FF951_RS10175 2181127..2181648 - 522 WP_003904745.1 hypothetical protein -
FF951_RS10180 (FF951_10510) 2181623..2182000 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
FF951_RS10185 (FF951_10515) 2182042..2182491 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
FF951_RS10190 (FF951_10520) 2182488..2183033 + 546 WP_003409891.1 SecB-like chaperone -
FF951_RS10195 (FF951_10525) 2182922..2183536 - 615 WP_003901296.1 hypothetical protein -
FF951_RS10200 (FF951_10530) 2183585..2183881 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
FF951_RS10205 (FF951_10535) 2183878..2184129 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
FF951_RS10210 (FF951_10540) 2184116..2184610 + 495 WP_003899099.1 hypothetical protein -
FF951_RS10215 (FF951_10545) 2184770..2185177 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
FF951_RS10220 (FF951_10550) 2185181..2185453 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FF951_RS10225 (FF951_10555) 2185486..2186706 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T126529 WP_010886136.1 NZ_CP040688:2181623-2182000 [Mycobacterium tuberculosis complex sp. N0052]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T126529 NZ_CP040688:2181623-2182000 [Mycobacterium tuberculosis complex sp. N0052]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT126529 WP_003409886.1 NZ_CP040688:2182042-2182491 [Mycobacterium tuberculosis complex sp. N0052]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT126529 NZ_CP040688:2182042-2182491 [Mycobacterium tuberculosis complex sp. N0052]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCATCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References