Detailed information of TA system TA126458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3281446..3282140
• Replicon: chromosome
• Accession: NZ_CP040667
• Organism: Escherichia coli strain S17-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: FGH86_RS16245
• Protein ID: WP_001263489.1
• Coordinates: 3281446..3281844 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FGH86_RS16250
• Protein ID: WP_000554758.1
• Coordinates: 3281847..3282140 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGH86_RS16215	3276491..3277618	-	1128	Protein_3149	esterase FrsA	-
-	3277034..3277114	-	81	NuclAT_12	-	-
-	3277034..3277114	-	81	NuclAT_12	-	-
-	3277034..3277114	-	81	NuclAT_12	-	-
-	3277034..3277114	-	81	NuclAT_12	-	-
FGH86_RS16220	3277710..3278168	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FGH86_RS16225	3278429..3279886	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
FGH86_RS16230	3279943..3280500	-	558	WP_001352051.1	peptide chain release factor H	-
FGH86_RS16235	3280412..3280678	-	267	WP_001295202.1	hypothetical protein	-
FGH86_RS16240	3280984..3281436	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
FGH86_RS16245	3281446..3281844	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FGH86_RS16250	3281847..3282140	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FGH86_RS16255	3282192..3283247	-	1056	WP_001226164.1	DNA polymerase IV	-
FGH86_RS16260	3283318..3284103	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
FGH86_RS16265	3284048..3285787	+	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
FGH86_RS16270	3286011..3286508	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T126458 WP_001263489.1 NZ_CP040667:c3281844-3281446 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T126458 NZ_CP040667:c3281844-3281446 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT126458 WP_000554758.1 NZ_CP040667:c3282140-3281847 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT126458 NZ_CP040667:c3282140-3281847 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3