Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1325596..1326098 Replicon chromosome
Accession NZ_CP040667
Organism Escherichia coli strain S17-1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FGH86_RS06220 Protein ID WP_000767829.1
Coordinates 1325844..1326098 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FGH86_RS06215 Protein ID WP_001259255.1
Coordinates 1325596..1325847 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGH86_RS06190 1320774..1321841 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FGH86_RS06195 1321841..1322911 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
FGH86_RS06200 1322908..1324212 - 1305 WP_000009594.1 histidinol dehydrogenase -
FGH86_RS06205 1324218..1325117 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FGH86_RS06210 1325263..1325313 - 51 WP_001364200.1 his operon leader peptide -
FGH86_RS06215 1325596..1325847 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FGH86_RS06220 1325844..1326098 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FGH86_RS06225 1326181..1327005 + 825 WP_000754737.1 SDR family oxidoreductase -
FGH86_RS06230 1327051..1327980 + 930 WP_000803351.1 LysR family transcriptional regulator -
FGH86_RS06235 1328195..1328257 + 63 WP_010723108.1 membrane protein YoeI -
FGH86_RS06240 1328247..1329605 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FGH86_RS06245 1329784..1330842 + 1059 WP_000492339.1 transport protein YeeE -
FGH86_RS06250 1330856..1331083 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T126444 WP_000767829.1 NZ_CP040667:1325844-1326098 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T126444 NZ_CP040667:1325844-1326098 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT126444 WP_001259255.1 NZ_CP040667:1325596-1325847 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT126444 NZ_CP040667:1325596-1325847 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References