Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2076490..2076992 Replicon chromosome
Accession NZ_CP040664
Organism Escherichia coli strain KR2009

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FE384_RS10370 Protein ID WP_000767829.1
Coordinates 2076490..2076744 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FE384_RS10375 Protein ID WP_001259255.1
Coordinates 2076741..2076992 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE384_RS10340 2071505..2071732 - 228 WP_000234896.1 sulfurtransferase TusA -
FE384_RS10345 2071746..2072804 - 1059 WP_000492339.1 transport protein YeeE -
FE384_RS10350 2072983..2074341 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FE384_RS10355 2074331..2074393 - 63 WP_010723108.1 membrane protein YoeI -
FE384_RS10360 2074608..2075537 - 930 WP_000803351.1 LysR family transcriptional regulator -
FE384_RS10365 2075583..2076407 - 825 WP_000754737.1 SDR family oxidoreductase -
FE384_RS10370 2076490..2076744 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FE384_RS10375 2076741..2076992 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FE384_RS10380 2077275..2077325 + 51 WP_001364200.1 his operon leader peptide -
FE384_RS10385 2077471..2078370 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FE384_RS10390 2078376..2079680 + 1305 WP_000009594.1 histidinol dehydrogenase -
FE384_RS10395 2079677..2080747 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
FE384_RS10400 2080747..2081814 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T126405 WP_000767829.1 NZ_CP040664:c2076744-2076490 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T126405 NZ_CP040664:c2076744-2076490 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT126405 WP_001259255.1 NZ_CP040664:c2076992-2076741 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT126405 NZ_CP040664:c2076992-2076741 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References