Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2078111..2078613 Replicon chromosome
Accession NZ_CP040663
Organism Escherichia coli strain EK2009

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FE383_RS10380 Protein ID WP_000767829.1
Coordinates 2078111..2078365 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FE383_RS10385 Protein ID WP_001259255.1
Coordinates 2078362..2078613 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE383_RS10350 2073126..2073353 - 228 WP_000234896.1 sulfurtransferase TusA -
FE383_RS10355 2073367..2074425 - 1059 WP_000492339.1 transport protein YeeE -
FE383_RS10360 2074604..2075962 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FE383_RS10365 2075952..2076014 - 63 WP_010723108.1 membrane protein YoeI -
FE383_RS10370 2076229..2077158 - 930 WP_000803351.1 LysR family transcriptional regulator -
FE383_RS10375 2077204..2078028 - 825 WP_000754737.1 SDR family oxidoreductase -
FE383_RS10380 2078111..2078365 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FE383_RS10385 2078362..2078613 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FE383_RS10390 2078896..2078946 + 51 WP_001364200.1 his operon leader peptide -
FE383_RS10395 2079092..2079991 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FE383_RS10400 2079997..2081301 + 1305 WP_000009594.1 histidinol dehydrogenase -
FE383_RS10405 2081298..2082368 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
FE383_RS10410 2082368..2083435 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T126362 WP_000767829.1 NZ_CP040663:c2078365-2078111 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T126362 NZ_CP040663:c2078365-2078111 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT126362 WP_001259255.1 NZ_CP040663:c2078613-2078362 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT126362 NZ_CP040663:c2078613-2078362 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References