Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3952822..3953419 Replicon chromosome
Accession NZ_CP040660
Organism Klebsiella pneumoniae strain 2019011308

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag FE373_RS19230 Protein ID WP_004142563.1
Coordinates 3953102..3953419 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag FE373_RS19225 Protein ID WP_004142561.1
Coordinates 3952822..3953109 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE373_RS19195 (3948894) 3948894..3949142 + 249 WP_020325243.1 DUF1158 domain-containing protein -
FE373_RS19200 (3949160) 3949160..3949501 - 342 WP_159164432.1 RamA family antibiotic efflux transcriptional regulator -
FE373_RS19205 (3949532) 3949532..3950647 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
FE373_RS19210 (3950827) 3950827..3951416 + 590 Protein_3769 TetR/AcrR family transcriptional regulator -
FE373_RS19215 (3951416) 3951416..3951784 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
FE373_RS19220 (3951904) 3951904..3952557 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
FE373_RS19225 (3952822) 3952822..3953109 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
FE373_RS19230 (3953102) 3953102..3953419 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FE373_RS19235 (3953604) 3953604..3954647 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
FE373_RS19240 (3955317) 3955317..3956183 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
FE373_RS19245 (3956292) 3956292..3957719 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T126328 WP_004142563.1 NZ_CP040660:c3953419-3953102 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T126328 NZ_CP040660:c3953419-3953102 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT126328 WP_004142561.1 NZ_CP040660:c3953109-3952822 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT126328 NZ_CP040660:c3953109-3952822 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References