Detailed information of TA system TA126247

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-ralA/-
• Location: 365444..365865
• Replicon: chromosome
• Accession: NZ_CP040643
• Organism: Escherichia coli strain BE104

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: FGC38_RS01810
• Protein ID: WP_001302184.1
• Coordinates: 365707..365865 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	365444..365642 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGC38_RS01785	361374..361913	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
FGC38_RS01790	361970..362203	+	234	WP_000005990.1	DUF905 family protein	-
FGC38_RS01795	362269..364227	+	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
FGC38_RS01800	364282..364716	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
FGC38_RS01805	364713..365432	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	365444..365642	+	199	NuclAT_1	-	Antitoxin
-	365444..365642	+	199	NuclAT_1	-	Antitoxin
-	365444..365642	+	199	NuclAT_1	-	Antitoxin
-	365444..365642	+	199	NuclAT_1	-	Antitoxin
FGC38_RS01810	365707..365865	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
FGC38_RS23345	366103..366462	-	360	Protein_344	hypothetical protein	-
FGC38_RS23350	366532..366738	+	207	WP_000547965.1	hypothetical protein	-
FGC38_RS01820	366763..367050	+	288	WP_000107542.1	hypothetical protein	-
FGC38_RS01825	367169..367990	+	822	WP_001234417.1	DUF945 domain-containing protein	-
FGC38_RS01830	368286..368888	-	603	WP_077763528.1	transglycosylase SLT domain-containing protein	-
FGC38_RS01835	369209..369592	+	384	WP_001151527.1	relaxosome protein TraM	-
FGC38_RS01840	369779..370468	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	277095..408551	131456

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T126247 WP_001302184.1 NZ_CP040643:365707-365865 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T126247 NZ_CP040643:365707-365865 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT126247 NZ_CP040643:365444-365642 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin