Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 252782..253476 Replicon chromosome
Accession NZ_CP040643
Organism Escherichia coli strain BE104

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag FGC38_RS01180 Protein ID WP_001263489.1
Coordinates 253078..253476 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag FGC38_RS01175 Protein ID WP_000554758.1
Coordinates 252782..253075 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGC38_RS01155 248414..248911 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
FGC38_RS01160 249135..250847 - 1713 Protein_227 flagellar biosynthesis protein FlhA -
FGC38_RS01165 250819..251604 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
FGC38_RS01170 251675..252730 + 1056 WP_001226164.1 DNA polymerase IV -
FGC38_RS01175 252782..253075 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FGC38_RS01180 253078..253476 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FGC38_RS01185 253486..253938 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
FGC38_RS01190 254256..254462 + 207 Protein_233 RtcB family protein -
FGC38_RS01195 254458..254979 + 522 Protein_234 peptide chain release factor H -
FGC38_RS01200 255036..256493 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
FGC38_RS01205 256754..257212 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 257808..257888 + 81 NuclAT_13 - -
- 257808..257888 + 81 NuclAT_13 - -
- 257808..257888 + 81 NuclAT_13 - -
- 257808..257888 + 81 NuclAT_13 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T126244 WP_001263489.1 NZ_CP040643:253078-253476 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T126244 NZ_CP040643:253078-253476 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT126244 WP_000554758.1 NZ_CP040643:252782-253075 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT126244 NZ_CP040643:252782-253075 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References