Detailed information of TA system TA126097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4381747..4382441
• Replicon: chromosome
• Accession: NZ_CP040572
• Organism: Escherichia coli O157:H7 strain ECP17-46

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: FGA21_RS22705
• Protein ID: WP_001263495.1
• Coordinates: 4381747..4382145 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FGA21_RS22710
• Protein ID: WP_000554757.1
• Coordinates: 4382148..4382441 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4377334..4377414	-	81	NuclAT_11	-	-
-	4377334..4377414	-	81	NuclAT_11	-	-
-	4377334..4377414	-	81	NuclAT_11	-	-
-	4377334..4377414	-	81	NuclAT_11	-	-
FGA21_RS22680	4378010..4378468	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FGA21_RS22685	4378729..4380186	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
FGA21_RS22690	4380243..4380764	-	522	Protein_4320	peptide chain release factor H	-
FGA21_RS22695	4380763..4380966	-	204	Protein_4321	RNA ligase RtcB family protein	-
FGA21_RS22700	4381285..4381737	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
FGA21_RS22705	4381747..4382145	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FGA21_RS22710	4382148..4382441	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FGA21_RS22715	4382493..4383548	-	1056	WP_001226186.1	DNA polymerase IV	-
FGA21_RS22720	4383619..4384404	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
FGA21_RS22725	4384376..4386088	+	1713	Protein_4327	flagellar biosynthesis protein FlhA	-
FGA21_RS22730	4386233..4386730	-	498	Protein_4328	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T126097 WP_001263495.1 NZ_CP040572:c4382145-4381747 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T126097 NZ_CP040572:c4382145-4381747 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT126097 WP_000554757.1 NZ_CP040572:c4382441-4382148 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT126097 NZ_CP040572:c4382441-4382148 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6