Detailed information of TA system TA126081

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2990487..2990712
• Replicon: chromosome
• Accession: NZ_CP040572
• Organism: Escherichia coli O157:H7 strain ECP17-46

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: FGA21_RS15580
• Protein ID: WP_000813258.1
• Coordinates: 2990487..2990642 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2990654..2990712 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGA21_RS15530	2985490..2985921	-	432	WP_001302123.1	tellurite resistance protein	-
FGA21_RS15545	2986372..2987085	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
FGA21_RS15550	2987221..2987418	-	198	WP_000917763.1	hypothetical protein	-
FGA21_RS15555	2987643..2988197	-	555	WP_000640035.1	DUF1133 family protein	-
FGA21_RS15560	2988260..2988565	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
FGA21_RS15565	2988578..2989627	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
FGA21_RS15570	2989629..2989901	-	273	WP_000191871.1	hypothetical protein	-
FGA21_RS15575	2990023..2990367	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
FGA21_RS15580	2990487..2990642	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2990654..2990712	+	59	-	-	Antitoxin
FGA21_RS15585	2990933..2991490	-	558	WP_000104474.1	DUF551 domain-containing protein	-
FGA21_RS15590	2991492..2991710	-	219	WP_000683609.1	DUF4014 family protein	-
FGA21_RS15595	2991838..2992149	-	312	WP_001289673.1	hypothetical protein	-
FGA21_RS15600	2992142..2992369	-	228	WP_000699809.1	hypothetical protein	-
FGA21_RS15605	2992366..2992647	-	282	WP_000603384.1	DNA-binding protein	-
FGA21_RS15610	2992680..2993396	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
FGA21_RS15615	2993430..2993891	-	462	WP_000139447.1	replication protein	-
FGA21_RS15620	2993884..2994939	-	1056	WP_001356791.1	hypothetical protein	-
FGA21_RS15625	2995008..2995433	-	426	WP_000693878.1	Rha family transcriptional regulator	-
FGA21_RS15630	2995417..2995660	-	244	Protein_2981	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2953041..3051201	98160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T126081 WP_000813258.1 NZ_CP040572:c2990642-2990487 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T126081 NZ_CP040572:c2990642-2990487 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT126081 NZ_CP040572:2990654-2990712 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin