Detailed information of TA system TA126059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4377369..4378063
• Replicon: chromosome
• Accession: NZ_CP040570
• Organism: Escherichia coli O157:H7 strain ECP17-1298

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: FGA23_RS22775
• Protein ID: WP_001263495.1
• Coordinates: 4377369..4377767 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FGA23_RS22780
• Protein ID: WP_000554757.1
• Coordinates: 4377770..4378063 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4372956..4373036	-	81	NuclAT_12	-	-
-	4372956..4373036	-	81	NuclAT_12	-	-
-	4372956..4373036	-	81	NuclAT_12	-	-
-	4372956..4373036	-	81	NuclAT_12	-	-
FGA23_RS22750	4373632..4374090	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FGA23_RS22755	4374351..4375808	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
FGA23_RS22760	4375865..4376386	-	522	Protein_4327	peptide chain release factor H	-
FGA23_RS22765	4376385..4376588	-	204	Protein_4328	RNA ligase RtcB family protein	-
FGA23_RS22770	4376907..4377359	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
FGA23_RS22775	4377369..4377767	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FGA23_RS22780	4377770..4378063	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FGA23_RS22785	4378115..4379170	-	1056	WP_001226188.1	DNA polymerase IV	-
FGA23_RS22790	4379241..4380026	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
FGA23_RS22795	4379998..4381710	+	1713	Protein_4334	flagellar biosynthesis protein FlhA	-
FGA23_RS22800	4381855..4382352	-	498	Protein_4335	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T126059 WP_001263495.1 NZ_CP040570:c4377767-4377369 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T126059 NZ_CP040570:c4377767-4377369 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT126059 WP_000554757.1 NZ_CP040570:c4378063-4377770 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT126059 NZ_CP040570:c4378063-4377770 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6