Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3213187..3213982 Replicon chromosome
Accession NZ_CP040570
Organism Escherichia coli O157:H7 strain ECP17-1298

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag FGA23_RS16805 Protein ID WP_000854914.1
Coordinates 3213187..3213561 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag FGA23_RS16810 Protein ID WP_001280954.1
Coordinates 3213608..3213982 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGA23_RS16765 3208191..3208682 - 492 WP_001301587.1 DUF1097 domain-containing protein -
FGA23_RS16770 3208784..3209338 - 555 WP_001001917.1 molecular chaperone YcdY -
FGA23_RS16775 3209362..3210099 - 738 WP_000283664.1 zinc-binding phosphatase -
FGA23_RS16780 3210154..3211092 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
FGA23_RS16790 3211563..3212405 - 843 WP_001280481.1 DUF4942 domain-containing protein -
FGA23_RS16795 3212490..3212687 - 198 WP_000839282.1 DUF957 domain-containing protein -
FGA23_RS16800 3212699..3213190 - 492 WP_000976853.1 hypothetical protein -
FGA23_RS16805 3213187..3213561 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
FGA23_RS16810 3213608..3213982 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
FGA23_RS16815 3214145..3214366 - 222 WP_001220314.1 DUF987 domain-containing protein -
FGA23_RS16820 3214429..3214905 - 477 WP_001186738.1 RadC family protein -
FGA23_RS16825 3214921..3215406 - 486 WP_000214398.1 antirestriction protein -
FGA23_RS16830 3215497..3216315 - 819 WP_001234682.1 DUF945 domain-containing protein -
FGA23_RS16840 3216655..3217725 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
FGA23_RS16845 3217722..3218627 - 906 WP_000203541.1 chemotaxis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3204573..3215406 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T126055 WP_000854914.1 NZ_CP040570:c3213561-3213187 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T126055 NZ_CP040570:c3213561-3213187 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT126055 WP_001280954.1 NZ_CP040570:c3213982-3213608 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT126055 NZ_CP040570:c3213982-3213608 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References