Detailed information of TA system TA126050

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2578036..2578407
• Replicon: chromosome
• Accession: NZ_CP040570
• Organism: Escherichia coli O157:H7 strain ECP17-1298

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: FGA23_RS13135
• Protein ID: WP_001443846.1
• Coordinates: 2578258..2578407 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2578036..2578214 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGA23_RS28360	2573788..2573958	+	171	WP_001625136.1	hypothetical protein	-
FGA23_RS13110	2573991..2575364	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
FGA23_RS13115	2575493..2576428	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
FGA23_RS13120	2576480..2577715	-	1236	WP_000040839.1	site-specific integrase	-
FGA23_RS13125	2577717..2577932	-	216	WP_000079604.1	excisionase XisR	-
FGA23_RS13130	2578032..2578220	-	189	WP_001302840.1	DUF1187 family protein	-
-	2578036..2578214	+	179	NuclAT_0	-	Antitoxin
-	2578036..2578214	+	179	NuclAT_0	-	Antitoxin
-	2578036..2578214	+	179	NuclAT_0	-	Antitoxin
-	2578036..2578214	+	179	NuclAT_0	-	Antitoxin
FGA23_RS13135	2578258..2578407	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
FGA23_RS13140	2578463..2579272	-	810	WP_000166313.1	recombination protein RecT	-
FGA23_RS13145	2579265..2581865	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
FGA23_RS13150	2581967..2582242	-	276	WP_001344816.1	hypothetical protein	-
FGA23_RS13155	2582317..2582487	-	171	WP_001352098.1	conserved protein, Rac prophage	-
FGA23_RS13160	2582487..2582708	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2563414..2680557	117143

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T126050 WP_001443846.1 NZ_CP040570:c2578407-2578258 [Escherichia coli O157:H7]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T126050 NZ_CP040570:c2578407-2578258 [Escherichia coli O157:H7]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT126050 NZ_CP040570:2578036-2578214 [Escherichia coli O157:H7]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin