Detailed information of TA system TA126000

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66909..67335
• Replicon: plasmid pCR-HvKP5-KPC
• Accession: NZ_CP040547
• Organism: Klebsiella pneumoniae strain CR-HvKP5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FFT49_RS28275
• Protein ID: WP_001312861.1
• Coordinates: 66909..67067 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	67111..67335 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFT49_RS28230	61964..62191	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
FFT49_RS28235	62285..62971	-	687	WP_015059009.1	PAS domain-containing protein	-
FFT49_RS28240	63162..63545	-	384	WP_000124981.1	relaxosome protein TraM	-
FFT49_RS28245	63822..64469	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
FFT49_RS28250	64766..65587	-	822	WP_001234445.1	DUF945 domain-containing protein	-
FFT49_RS28255	65698..65994	-	297	WP_001272251.1	hypothetical protein	-
FFT49_RS30610	66294..66671	+	378	Protein_83	hypothetical protein	-
FFT49_RS28275	66909..67067	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	67111..67335	-	225	NuclAT_0	-	Antitoxin
-	67111..67335	-	225	NuclAT_0	-	Antitoxin
-	67111..67335	-	225	NuclAT_0	-	Antitoxin
-	67111..67335	-	225	NuclAT_0	-	Antitoxin
FFT49_RS28280	67347..68066	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
FFT49_RS28285	68063..68497	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FFT49_RS28290	68566..70589	-	2024	Protein_87	ParB/RepB/Spo0J family partition protein	-
FFT49_RS28295	70650..70883	-	234	WP_000006003.1	DUF905 family protein	-
FFT49_RS28300	70941..71468	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
FFT49_RS30615	71770..72225	+	456	Protein_90	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 / catA2	-	1..177587	177587

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T126000 WP_001312861.1 NZ_CP040547:c67067-66909 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T126000 NZ_CP040547:c67067-66909 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT126000 NZ_CP040547:c67335-67111 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin