Detailed information of TA system TA125998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 81215..81942
• Replicon: plasmid pCR-HvKP5-VIR
• Accession: NZ_CP040546
• Organism: Klebsiella pneumoniae strain CR-HvKP5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: FFT49_RS27280
• Protein ID: WP_011251285.1
• Coordinates: 81631..81942 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FFT49_RS27275
• Protein ID: WP_011251286.1
• Coordinates: 81215..81634 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFT49_RS27240	76669..77907	+	1239	Protein_76	copper/silver sensor histidine kinase SilS	-
FFT49_RS27250	77940..78908	+	969	WP_004225014.1	IS5 family transposase	-
FFT49_RS27255	78942..79649	+	708	WP_094819173.1	hypothetical protein	-
FFT49_RS27260	79663..79998	+	336	Protein_79	conjugal transfer protein TraI	-
FFT49_RS27265	80100..81068	-	969	WP_077270001.1	IS5 family transposase	-
FFT49_RS27275	81215..81634	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
FFT49_RS27280	81631..81942	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
FFT49_RS27285	82147..82605	-	459	Protein_83	DDE-type integrase/transposase/recombinase	-
FFT49_RS27290	82719..83416	+	698	Protein_84	IS1 family transposase	-
FFT49_RS27295	83433..83867	+	435	Protein_85	thioredoxin fold domain-containing protein	-
FFT49_RS27300	83884..84195	+	312	WP_011251282.1	hypothetical protein	-
FFT49_RS27305	84209..84550	+	342	WP_011251281.1	hypothetical protein	-
FFT49_RS27310	84610..85566	+	957	WP_011251280.1	DsbA family protein	-
FFT49_RS27315	85683..86258	+	576	WP_074424927.1	hypothetical protein	-
FFT49_RS27320	86366..86881	+	516	WP_094819131.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA / rmpA2	1..177694	177694
	-	inside	IScluster/Tn	-	-	77940..83416	5476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T125998 WP_011251285.1 NZ_CP040546:c81942-81631 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T125998 NZ_CP040546:c81942-81631 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT125998 WP_011251286.1 NZ_CP040546:c81634-81215 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT125998 NZ_CP040546:c81634-81215 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1