Detailed information of TA system TA125974

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53343..53607
• Replicon: plasmid pCR-HvKP4-p3
• Accession: NZ_CP040542
• Organism: Klebsiella pneumoniae strain CR-HvKP4

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: FFT48_RS29525
• Protein ID: WP_001303307.1
• Coordinates: 53455..53607 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	53343..53405 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFT48_RS29510	49445..50515	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
FFT48_RS29515	50534..51742	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	51922..51982	-	61	NuclAT_1	-	-
-	51922..51982	-	61	NuclAT_1	-	-
-	51922..51982	-	61	NuclAT_1	-	-
-	51922..51982	-	61	NuclAT_1	-	-
FFT48_RS29520	52049..53140	-	1092	WP_000426061.1	hypothetical protein	-
-	53343..53405	-	63	NuclAT_0	-	Antitoxin
-	53343..53405	-	63	NuclAT_0	-	Antitoxin
-	53343..53405	-	63	NuclAT_0	-	Antitoxin
-	53343..53405	-	63	NuclAT_0	-	Antitoxin
FFT48_RS29525	53455..53607	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
FFT48_RS29530	53679..53930	-	252	WP_001291968.1	hypothetical protein	-
-	54317..54368	-	52	NuclAT_2	-	-
-	54317..54368	-	52	NuclAT_2	-	-
-	54317..54368	-	52	NuclAT_2	-	-
-	54317..54368	-	52	NuclAT_2	-	-
FFT48_RS30625	54854..55030	-	177	WP_001054900.1	hypothetical protein	-
FFT48_RS29545	55239..55448	-	210	WP_001140545.1	hemolysin expression modulator Hha	-
FFT48_RS29550	55546..56160	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
FFT48_RS29555	56236..58404	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..99722	99722

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T125974 WP_001303307.1 NZ_CP040542:53455-53607 [Klebsiella pneumoniae]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T125974 NZ_CP040542:53455-53607 [Klebsiella pneumoniae]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT125974 NZ_CP040542:c53405-53343 [Klebsiella pneumoniae]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin