Detailed information of TA system TA125966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 62200..62927
• Replicon: plasmid pCR-HvKP4-VIR
• Accession: NZ_CP040540
• Organism: Klebsiella pneumoniae strain CR-HvKP4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: FFT48_RS27100
• Protein ID: WP_011251285.1
• Coordinates: 62616..62927 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FFT48_RS27095
• Protein ID: WP_011251286.1
• Coordinates: 62200..62619 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFT48_RS27060	57654..58892	+	1239	Protein_54	copper/silver sensor histidine kinase SilS	-
FFT48_RS27070	58925..59893	+	969	WP_004225014.1	IS5 family transposase	-
FFT48_RS27075	59927..60634	+	708	WP_094819173.1	hypothetical protein	-
FFT48_RS27080	60648..60983	+	336	Protein_57	conjugal transfer protein TraI	-
FFT48_RS27085	61085..62053	-	969	WP_077270001.1	IS5 family transposase	-
FFT48_RS27095	62200..62619	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
FFT48_RS27100	62616..62927	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
FFT48_RS27105	63132..63590	-	459	Protein_61	DDE-type integrase/transposase/recombinase	-
FFT48_RS27110	63704..64401	+	698	Protein_62	IS1 family transposase	-
FFT48_RS27115	64418..64852	+	435	Protein_63	thioredoxin fold domain-containing protein	-
FFT48_RS27120	64869..65180	+	312	WP_011251282.1	hypothetical protein	-
FFT48_RS27125	65194..65535	+	342	WP_011251281.1	hypothetical protein	-
FFT48_RS27130	65595..66551	+	957	WP_011251280.1	DsbA family protein	-
FFT48_RS27135	66668..67243	+	576	WP_074424927.1	hypothetical protein	-
FFT48_RS27140	67351..67866	+	516	WP_094819131.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA	1..178154	178154
	-	inside	IScluster/Tn	-	-	58925..64401	5476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T125966 WP_011251285.1 NZ_CP040540:c62927-62616 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T125966 NZ_CP040540:c62927-62616 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT125966 WP_011251286.1 NZ_CP040540:c62619-62200 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT125966 NZ_CP040540:c62619-62200 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1