Detailed information of TA system TA125938

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 104270..104540
• Replicon: plasmid pCR-HvKP1-KPC
• Accession: NZ_CP040535
• Organism: Klebsiella pneumoniae strain CR-HvKP1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FFT47_RS28630
• Protein ID: WP_001312861.1
• Coordinates: 104382..104540 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	104270..104333 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFT47_RS28605	99981..100508	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
FFT47_RS28610	100566..100799	+	234	WP_000006003.1	DUF905 family protein	-
FFT47_RS28615	100860..102883	+	2024	Protein_141	ParB/RepB/Spo0J family partition protein	-
FFT47_RS28620	102952..103386	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FFT47_RS28625	103383..104102	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	104114..104338	+	225	NuclAT_0	-	-
-	104114..104338	+	225	NuclAT_0	-	-
-	104114..104338	+	225	NuclAT_0	-	-
-	104114..104338	+	225	NuclAT_0	-	-
-	104270..104333	-	64	-	-	Antitoxin
FFT47_RS28630	104382..104540	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FFT47_RS30540	104778..105155	-	378	Protein_145	hypothetical protein	-
FFT47_RS28650	105455..105751	+	297	WP_001272251.1	hypothetical protein	-
FFT47_RS28655	105862..106683	+	822	WP_001234445.1	DUF945 domain-containing protein	-
FFT47_RS28660	106980..107627	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
FFT47_RS28665	107904..108287	+	384	WP_000124981.1	relaxosome protein TraM	-
FFT47_RS28670	108478..109164	+	687	WP_015059009.1	PAS domain-containing protein	-
FFT47_RS28675	109258..109485	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA2 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2	-	1..177591	177591

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T125938 WP_001312861.1 NZ_CP040535:104382-104540 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T125938 NZ_CP040535:104382-104540 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT125938 NZ_CP040535:c104333-104270 [Klebsiella pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin