Detailed information of TA system TA125880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1377598..1378430
• Replicon: chromosome
• Accession: NZ_CP040511
• Organism: Acidithiobacillus ferrooxidans strain YNTRS-40

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: B7J9L0
• Locus tag: FE661_RS07130
• Protein ID: WP_009569577.1
• Coordinates: 1377598..1378104 (-)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: B7J9L1
• Locus tag: FE661_RS07135
• Protein ID: WP_009569578.1
• Coordinates: 1378101..1378430 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE661_RS07100	1372606..1372995	+	390	WP_009565421.1	holo-ACP synthase	-
FE661_RS07105	1372999..1374321	+	1323	WP_012607056.1	UDP-glucose/GDP-mannose dehydrogenase family protein	-
FE661_RS07110	1374302..1375273	+	972	WP_049759457.1	D-glycero-beta-D-manno-heptose-7-phosphate kinase	-
FE661_RS07115	1375275..1376045	+	771	WP_012536502.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
FE661_RS07120	1376038..1376880	+	843	WP_012536503.1	Hsp33 family molecular chaperone HslO	-
FE661_RS07130	1377598..1378104	-	507	WP_009569577.1	type II toxin-antitoxin system YhaV family toxin	Toxin
FE661_RS07135	1378101..1378430	-	330	WP_009569578.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
FE661_RS07140	1378717..1378860	+	144	WP_009569579.1	hypothetical protein	-
FE661_RS07145	1378887..1379153	-	267	WP_012536504.1	type I restriction-modification system subunit M N-terminal domain-containing protein	-
FE661_RS07150	1379498..1379992	-	495	WP_009565062.1	GNAT family N-acetyltransferase	-
FE661_RS07155	1379989..1380261	-	273	WP_012536505.1	DUF1778 domain-containing protein	-
FE661_RS07160	1380342..1380554	-	213	WP_009562915.1	hypothetical protein	-
FE661_RS07165	1380722..1380938	-	217	Protein_1407	hypothetical protein	-
FE661_RS07170	1381338..1382159	-	822	WP_012536506.1	UDP-2,3-diacylglucosamine diphosphatase	-
FE661_RS07175	1382400..1382645	+	246	WP_009568482.1	DUF2934 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (16-152)

Antitoxin

• PrlF antitoxin (8-107)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 19373.17 Da
• Isoelectric Point: 9.9366

>T125880 WP_009569577.1 NZ_CP040511:c1378104-1377598 [Acidithiobacillus ferrooxidans]
MSSSKLAPLVIHGWTVFAHPLFLAHIETLAQQVEAFKKKDPVGYVKKNANKRLAAIIKLAFDAIPQDPTRPEYRQGNTLG
EDHKHWFRAKFFQQYRLFFRYHAPSKVIVLAWVNDEDTKRAYESSDDAYRVFRKMLESGRPPDDWNQLLAEACAEGQRLQ
RFAAGIAP

Nucleotide

• Length: 507 bp

>T125880 NZ_CP040511:c1378104-1377598 [Acidithiobacillus ferrooxidans]
ATGAGCTCGAGCAAGCTCGCGCCCTTGGTCATTCATGGCTGGACGGTTTTTGCCCACCCGCTATTCCTCGCGCATATTGA
GACCTTGGCCCAGCAGGTCGAGGCCTTCAAGAAAAAGGATCCCGTCGGATACGTGAAAAAGAACGCCAATAAGCGACTGG
CGGCGATCATCAAACTGGCGTTCGACGCCATCCCGCAAGACCCGACACGGCCAGAGTACCGTCAAGGCAACACCCTCGGT
GAAGATCACAAGCACTGGTTCCGCGCCAAGTTTTTCCAGCAATACCGGCTGTTCTTCCGCTACCACGCGCCGAGCAAGGT
GATCGTGCTCGCCTGGGTCAATGACGAGGACACCAAGCGCGCCTATGAGAGCAGTGACGACGCCTATCGGGTGTTCCGAA
AGATGCTGGAAAGCGGCCGTCCTCCGGATGACTGGAACCAGTTGCTGGCCGAGGCATGCGCCGAGGGGCAGCGACTACAG
CGATTCGCTGCTGGCATTGCACCGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12107.54 Da
• Isoelectric Point: 4.3141

>AT125880 WP_009569578.1 NZ_CP040511:c1378430-1378101 [Acidithiobacillus ferrooxidans]
MAATLEVESTLTDRYQTTVPETVRRALRLGKRDKIHYTIRPGGEVVLTRAEDSEEDDPVLGQFLGFLARDIATHPERLQA
IDVSFVQRLQSLTGGIEVDLDAPLSADDE

Nucleotide

• Length: 330 bp

>AT125880 NZ_CP040511:c1378430-1378101 [Acidithiobacillus ferrooxidans]
ATGGCTGCCACCCTCGAAGTCGAATCCACGCTGACTGACCGCTACCAAACCACGGTGCCGGAGACCGTCCGCCGGGCCCT
CCGGCTGGGCAAGCGCGACAAGATCCACTACACCATCCGCCCCGGTGGCGAGGTCGTGCTGACGCGCGCCGAGGACTCCG
AGGAAGACGATCCGGTGCTCGGTCAGTTCCTGGGCTTCCTGGCCCGCGACATCGCCACGCATCCAGAGCGCCTGCAGGCC
ATCGATGTCAGCTTCGTGCAGCGCCTCCAATCGCTGACCGGCGGCATCGAAGTCGATCTCGATGCCCCCTTGTCTGCGGA
CGATGAATGA

Structures

Toxin

• AlphaFold DB: B7J9L0

Antitoxin

• AlphaFold DB: B7J9L1