Detailed information of TA system TA125874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 87242..87850
• Replicon: chromosome
• Accession: NZ_CP040511
• Organism: Acidithiobacillus ferrooxidans strain YNTRS-40

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2W1K1W3
• Locus tag: FE661_RS00440
• Protein ID: WP_012535782.1
• Coordinates: 87242..87517 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: FE661_RS00445
• Protein ID: WP_009566298.1
• Coordinates: 87533..87850 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE661_RS00405	83020..84060	+	1041	WP_012535779.1	glycosyltransferase family 4 protein	-
FE661_RS00410	84128..85054	+	927	WP_012535780.1	glycosyltransferase family 2 protein	-
FE661_RS00415	85059..85415	+	357	Protein_82	glycosyltransferase	-
FE661_RS00425	86155..86376	+	222	WP_009566302.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FE661_RS00430	86376..86789	+	414	WP_009566301.1	type II toxin-antitoxin system VapC family toxin	-
FE661_RS00435	86786..87076	+	291	WP_009566300.1	hypothetical protein	-
FE661_RS00440	87242..87517	+	276	WP_012535782.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FE661_RS00445	87533..87850	+	318	WP_009566298.1	HigA family addiction module antidote protein	Antitoxin
FE661_RS00450	87850..87990	+	141	Protein_88	HNH endonuclease	-
FE661_RS00455	88464..89627	+	1164	WP_009566294.1	Fic family protein	-
FE661_RS00460	89760..90187	-	428	Protein_90	type II toxin-antitoxin system VapC family toxin	-
FE661_RS00465	90187..90429	-	243	WP_012535785.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FE661_RS00470	90513..90686	-	174	WP_009562770.1	PCP reductase family protein	-
FE661_RS00475	90797..91225	-	429	WP_009562768.1	hypothetical protein	-
FE661_RS00480	91228..91659	-	432	WP_009562767.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10433.84 Da
• Isoelectric Point: 7.9070

>T125874 WP_012535782.1 NZ_CP040511:87242-87517 [Acidithiobacillus ferrooxidans]
MIRSFVHKGLKQVFETGQSAKVGADLVTRCKRRLDAINAATSPEELNVPGFDFHALEGKPKRYTVHVNGPYCITFEWREG
DAWRIDLENYH

Nucleotide

• Length: 276 bp

>T125874 NZ_CP040511:87242-87517 [Acidithiobacillus ferrooxidans]
TTGATACGAAGTTTTGTGCATAAGGGCCTCAAGCAGGTTTTCGAAACAGGTCAATCGGCGAAAGTCGGTGCGGATTTGGT
AACCCGCTGCAAGCGCAGGCTCGATGCCATAAACGCTGCGACATCCCCGGAGGAGTTGAACGTGCCGGGTTTCGACTTCC
ATGCTTTGGAGGGGAAACCGAAGCGGTACACGGTTCATGTGAATGGACCCTATTGCATCACCTTCGAGTGGCGTGAAGGA
GATGCCTGGCGCATTGATCTGGAGAATTACCATTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11756.54 Da
• Isoelectric Point: 5.6631

>AT125874 WP_009566298.1 NZ_CP040511:87533-87850 [Acidithiobacillus ferrooxidans]
MDEMTVEQVDRIPTHPGEILREEVLPALDISVAEFARGLRVSRQTVHGILSERKGITPEMALRIGKYLGNGPGIWLRMQQ
AYDLTVAERRLAEELAAIHKPEQAA

Nucleotide

• Length: 318 bp

>AT125874 NZ_CP040511:87533-87850 [Acidithiobacillus ferrooxidans]
ATGGACGAAATGACCGTTGAGCAAGTGGATCGGATACCGACGCATCCTGGCGAGATATTGCGGGAAGAGGTACTGCCCGC
ACTGGATATCTCTGTAGCAGAGTTTGCCCGTGGCCTGCGGGTCTCGCGCCAGACCGTTCACGGCATCCTGTCTGAGCGCA
AGGGCATCACCCCGGAGATGGCCTTGCGTATCGGCAAATATCTGGGTAACGGGCCGGGGATATGGCTACGCATGCAGCAG
GCTTACGATTTGACCGTTGCAGAACGTCGGTTGGCCGAAGAACTGGCGGCCATTCACAAGCCCGAGCAGGCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A2W1K1W3