Detailed information of TA system TA125867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5021174..5021936
• Replicon: chromosome
• Accession: NZ_CP040456
• Organism: Escherichia coli strain UPEC132

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0K5JE43
• Locus tag: FE252_RS26145
• Protein ID: WP_028125934.1
• Coordinates: 5021174..5021464 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FE252_RS26150
• Protein ID: WP_001534936.1
• Coordinates: 5021481..5021936 (+)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE252_RS26120	5017881..5018783	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FE252_RS26125	5018780..5019415	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FE252_RS26130	5019412..5020341	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
FE252_RS26135	5020557..5020775	-	219	WP_001468130.1	ribbon-helix-helix domain-containing protein	-
FE252_RS26145	5021174..5021464	+	291	WP_028125934.1	type II toxin-antitoxin system HigB family toxin	Toxin
FE252_RS26150	5021481..5021936	+	456	WP_001534936.1	helix-turn-helix domain-containing protein	Antitoxin
FE252_RS26155	5021947..5022888	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FE252_RS26160	5022933..5023370	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FE252_RS26165	5023367..5024239	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FE252_RS26170	5024233..5024832	-	600	WP_001443191.1	glucose-1-phosphatase	-
FE252_RS26175	5024973..5025860	-	888	WP_001298595.1	aldose 1-epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-148)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11608.45 Da
• Isoelectric Point: 8.8235

>T125867 WP_028125934.1 NZ_CP040456:5021174-5021464 [Escherichia coli]
MHVISHKPFNEAIKRFPPYEGALIDLLNLLEKTEFSDPGEMKRYIPSLDNFKYRDKWWVINIAGNHLRLIAYIDFRLQKC
FIKHTVIYNEYDRFTT

Nucleotide

• Length: 291 bp

>T125867 NZ_CP040456:5021174-5021464 [Escherichia coli]
ATGCATGTCATCAGCCACAAACCTTTTAACGAGGCGATAAAACGCTTTCCACCTTACGAAGGTGCACTTATTGATTTATT
AAATCTTCTTGAGAAAACAGAGTTTAGCGATCCGGGTGAGATGAAGAGATATATTCCCTCACTGGATAATTTTAAATACA
GAGATAAATGGTGGGTTATTAATATTGCAGGTAATCATTTACGGCTTATCGCATATATTGATTTCAGGCTCCAAAAATGT
TTTATCAAACATACTGTTATCTACAATGAATACGACAGATTCACAACCTAG

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 17121.55 Da
• Isoelectric Point: 6.6011

>AT125867 WP_001534936.1 NZ_CP040456:5021481-5021936 [Escherichia coli]
MRTHHTPAEDSANFGMLVKTFTHAVKDIPLCGPKADQEEYHNALKLIEFLVDRDDLENPLFELLCVRIREYENNAPEFQH
FNQRLASTPCGVSLLRILMDQHGLKAADLAQELGSKSNVSNILNGRRALTVRHIKALSERFNLPAEAFIDK

Nucleotide

• Length: 456 bp

>AT125867 NZ_CP040456:5021481-5021936 [Escherichia coli]
ATGAGAACCCATCACACCCCAGCAGAAGACTCGGCCAATTTTGGAATGCTGGTTAAGACCTTCACCCACGCAGTGAAAGA
CATCCCTTTATGTGGCCCTAAAGCGGATCAAGAGGAATACCATAATGCGCTGAAACTGATCGAATTTTTAGTCGATCGTG
ACGATCTGGAAAACCCTTTATTTGAATTACTCTGCGTGCGGATCCGCGAATATGAAAATAACGCTCCAGAATTTCAGCAT
TTTAATCAGAGATTAGCATCGACCCCGTGCGGTGTAAGTCTGTTACGGATCCTTATGGATCAACATGGGCTTAAAGCGGC
TGATTTGGCACAAGAACTTGGCTCTAAATCTAATGTGAGTAATATTCTTAATGGTCGCCGGGCGCTCACCGTTCGCCATA
TCAAGGCGTTAAGCGAGCGTTTTAATCTCCCGGCTGAAGCGTTTATTGATAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0K5JE43