Detailed information of TA system TA125832

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83515..83928
• Replicon: plasmid unnamed1
• Accession: NZ_CP040454
• Organism: Escherichia coli strain UPEC132

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: FE252_RS00535
• Protein ID: WP_000775238.1
• Coordinates: 83515..83676 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	83728..83928 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE252_RS00475	78871..79518	+	648	WP_139510591.1	transglycosylase SLT domain-containing protein	-
FE252_RS00480	79815..80636	-	822	WP_001234445.1	DUF945 domain-containing protein	-
FE252_RS00485	80746..81042	-	297	WP_001272251.1	hypothetical protein	-
FE252_RS27185	81513..81779	-	267	WP_186552645.1	hypothetical protein	-
FE252_RS00505	82045..82287	-	243	WP_000540588.1	hypothetical protein	-
FE252_RS00510	82346..82669	-	324	WP_000533253.1	hypothetical protein	-
FE252_RS00530	82965..83277	+	313	Protein_89	hypothetical protein	-
FE252_RS00535	83515..83676	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	83728..83928	-	201	NuclAT_0	-	Antitoxin
-	83728..83928	-	201	NuclAT_0	-	Antitoxin
-	83728..83928	-	201	NuclAT_0	-	Antitoxin
-	83728..83928	-	201	NuclAT_0	-	Antitoxin
FE252_RS27190	83885..84055	-	171	WP_186552646.1	hypothetical protein	-
FE252_RS00545	84128..84778	+	651	WP_032340503.1	IS66 family insertion sequence hypothetical protein	-
FE252_RS00550	84778..85125	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
FE252_RS00555	85145..86716	+	1572	WP_000381416.1	IS66 family transposase	-
FE252_RS00560	86747..86911	-	165	Protein_95	theronine dehydrogenase	-
FE252_RS00565	86908..87627	-	720	WP_020246013.1	plasmid SOS inhibition protein A	-
FE252_RS00570	87624..88058	-	435	WP_000845950.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..117139	117139

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T125832 WP_000775238.1 NZ_CP040454:c83676-83515 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T125832 NZ_CP040454:c83676-83515 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 201 bp

>AT125832 NZ_CP040454:c83928-83728 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATGAGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCTA

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin