Detailed information of TA system TA125828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3773027..3773661
• Replicon: chromosome
• Accession: NZ_CP040449
• Organism: Aeromonas simiae strain A6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5J6X1V1
• Locus tag: FE240_RS17970
• Protein ID: WP_069140143.1
• Coordinates: 3773368..3773661 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FE240_RS17965
• Protein ID: WP_074561555.1
• Coordinates: 3773027..3773353 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE240_RS17955	3768967..3772119	-	3153	WP_193002823.1	DEAD/DEAH box helicase	-
FE240_RS17960	3772109..3772957	-	849	WP_057108201.1	DUF1837 domain-containing protein	-
FE240_RS17965	3773027..3773353	-	327	WP_074561555.1	HigA family addiction module antidote protein	Antitoxin
FE240_RS17970	3773368..3773661	-	294	WP_069140143.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FE240_RS17975	3773791..3775002	-	1212	WP_069140142.1	site-specific integrase	-
FE240_RS17980	3775174..3776037	-	864	WP_042047653.1	YicC family protein	-
FE240_RS17985	3776187..3776903	+	717	WP_193002824.1	ribonuclease PH	-
FE240_RS17990	3776983..3777624	+	642	WP_193002825.1	orotate phosphoribosyltransferase	-
FE240_RS17995	3777688..3778182	+	495	WP_193002826.1	YbaK/prolyl-tRNA synthetase associated domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ampH	-	3680030..3803048	123018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11443.86 Da
• Isoelectric Point: 5.1406

>T125828 WP_069140143.1 NZ_CP040449:c3773661-3773368 [Aeromonas simiae]
MAVEFRDTWLEAFYEDDQSHRRIPSTIEGALFRKLEILDAATQESDLRIPPGNRFEHLEGNMAGWCSIRVNKQYRLIFRW
IDGVAQDTYLDPHVYRG

Nucleotide

• Length: 294 bp

>T125828 NZ_CP040449:c3773661-3773368 [Aeromonas simiae]
ATGGCTGTCGAGTTTAGAGACACGTGGCTGGAAGCGTTTTACGAGGATGACCAAAGCCACAGGAGGATACCTAGTACCAT
CGAAGGTGCATTGTTCAGGAAGCTGGAGATCTTGGACGCAGCGACCCAGGAGTCAGACTTGAGAATCCCTCCGGGTAACC
GCTTCGAGCACCTTGAAGGCAATATGGCCGGGTGGTGTTCCATCCGGGTAAACAAGCAATACCGGCTGATCTTCAGATGG
ATCGATGGCGTTGCCCAAGATACTTATCTGGATCCTCATGTTTACAGGGGCTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12362.35 Da
• Isoelectric Point: 10.4719

>AT125828 WP_074561555.1 NZ_CP040449:c3773353-3773027 [Aeromonas simiae]
MRNTKRRPVTVGQMLVTEFLEPMNIEINELAEAMGVHRNTLSRIVHDKGALTAPMSIKLAAALGNTPEFWLNIQHSVELW
DVRHRAFEQEAKNVKRVTPHLKNREAVI

Nucleotide

• Length: 327 bp

>AT125828 NZ_CP040449:c3773353-3773027 [Aeromonas simiae]
ATGAGAAACACAAAACGTCGCCCAGTTACGGTCGGTCAGATGCTGGTAACCGAGTTCCTGGAGCCTATGAACATCGAGAT
CAACGAACTGGCTGAAGCCATGGGTGTGCACAGAAACACCCTGAGTCGGATCGTTCACGACAAGGGGGCATTGACCGCAC
CCATGTCAATCAAACTGGCAGCGGCCTTGGGTAACACGCCGGAGTTCTGGCTCAATATTCAGCACTCAGTAGAGTTGTGG
GATGTTCGGCATAGAGCCTTTGAGCAGGAAGCAAAGAATGTTAAACGAGTAACGCCTCACTTGAAGAACAGGGAAGCAGT
TATTTAG

Structures

Toxin

• AlphaFold DB: A0A5J6X1V1