Detailed information of TA system TA125817

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4485355..4485726
• Replicon: chromosome
• Accession: NZ_CP040443
• Organism: Escherichia sp. E4742

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: FEM44_RS21780
• Protein ID: WP_001317028.1
• Coordinates: 4485532..4485726 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4485355..4485533 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEM44_RS21755	4481312..4482685	+	1374	WP_130216112.1	ATP-dependent RNA helicase DbpA	-
FEM44_RS21760	4482812..4483747	-	936	WP_135486172.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
FEM44_RS21765	4483799..4485034	-	1236	WP_135522677.1	site-specific integrase	-
FEM44_RS21770	4485036..4485251	-	216	WP_000079604.1	excisionase XisR	-
FEM44_RS21775	4485351..4485539	-	189	WP_001302840.1	DUF1187 family protein	-
-	4485355..4485533	+	179	NuclAT_0	-	Antitoxin
-	4485355..4485533	+	179	NuclAT_0	-	Antitoxin
-	4485355..4485533	+	179	NuclAT_0	-	Antitoxin
-	4485355..4485533	+	179	NuclAT_0	-	Antitoxin
FEM44_RS21780	4485532..4485726	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
FEM44_RS21785	4485783..4486592	-	810	WP_032317649.1	recombination protein RecT	-
FEM44_RS21790	4486585..4489266	-	2682	WP_135522676.1	exodeoxyribonuclease VIII	-
FEM44_RS21795	4489368..4489643	-	276	WP_138159182.1	hypothetical protein	-
FEM44_RS21800	4489718..4489918	-	201	WP_135522675.1	hypothetical protein	-
FEM44_RS21805	4489848..4490084	-	237	WP_135522674.1	DUF1482 family protein	-
FEM44_RS21810	4490005..4490214	-	210	WP_000440741.1	host cell division inhibitory peptide Kil	-
FEM44_RS21815	4490233..4490505	-	273	WP_001156287.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4483799..4532762	48963

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T125817 WP_001317028.1 NZ_CP040443:c4485726-4485532 [Escherichia sp. E4742]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T125817 NZ_CP040443:c4485726-4485532 [Escherichia sp. E4742]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGGAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT125817 NZ_CP040443:4485355-4485533 [Escherichia sp. E4742]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCTCCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin