Detailed information of TA system TA125752

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 93570..93809
• Replicon: plasmid pCTX-M-15_22372
• Accession: NZ_CP040398
• Organism: Escherichia coli strain BA22372

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: FEV15_RS23795
• Protein ID: WP_023144756.1
• Coordinates: 93675..93809 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	93570..93630 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEV15_RS23765	88687..91080	+	2394	Protein_101	AAA family ATPase	-
FEV15_RS23770	91100..91846	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
FEV15_RS23775	91901..92461	+	561	WP_000139341.1	fertility inhibition protein FinO	-
FEV15_RS23780	92592..92804	+	213	WP_013023861.1	hypothetical protein	-
FEV15_RS23790	93317..93603	+	287	Protein_105	DUF2726 domain-containing protein	-
-	93570..93630	-	61	NuclAT_0	-	Antitoxin
-	93570..93630	-	61	NuclAT_0	-	Antitoxin
-	93570..93630	-	61	NuclAT_0	-	Antitoxin
-	93570..93630	-	61	NuclAT_0	-	Antitoxin
FEV15_RS23795	93675..93809	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
FEV15_RS23800	94106..94360	+	255	WP_000083850.1	replication regulatory protein RepA	-
FEV15_RS23805	94520..95265	-	746	Protein_108	IS21-like element ISEc12 family helper ATPase IstB	-
FEV15_RS23810	95280..96821	-	1542	WP_138223048.1	IS21-like element ISEc12 family transposase	-
FEV15_RS24550	97052..97186	+	135	Protein_110	protein CopA/IncA	-
FEV15_RS23820	97183..97257	+	75	WP_031943482.1	RepA leader peptide Tap	-
FEV15_RS23825	97250..98107	+	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / mph(A) / aac(3)-IId / aadA5 / qacE / sul1 / tet(B)	-	1..98470	98470

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T125752 WP_023144756.1 NZ_CP040398:93675-93809 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T125752 NZ_CP040398:93675-93809 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT125752 NZ_CP040398:c93630-93570 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin