Detailed information of TA system TA125744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4283552..4284279
• Replicon: chromosome
• Accession: NZ_CP040397
• Organism: Escherichia coli strain BA22372

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: FEV15_RS20855
• Protein ID: WP_000547564.1
• Coordinates: 4283968..4284279 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FEV15_RS20850
• Protein ID: WP_000126294.1
• Coordinates: 4283552..4283971 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEV15_RS20830	4278603..4279130	-	528	WP_001078777.1	electron transport protein HydN	-
FEV15_RS20835	4279278..4280288	-	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
FEV15_RS20840	4280548..4282005	+	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FEV15_RS20845	4282014..4283438	+	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
FEV15_RS20850	4283552..4283971	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
FEV15_RS20855	4283968..4284279	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
FEV15_RS20860	4284453..4285214	-	762	WP_001026446.1	hypothetical protein	-
FEV15_RS20865	4285239..4285709	-	471	WP_060621146.1	hydrogenase maturation peptidase HycI	-
FEV15_RS20870	4285702..4286112	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
FEV15_RS20875	4286109..4286876	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
FEV15_RS20880	4286876..4287418	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
FEV15_RS20885	4287428..4289137	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T125744 WP_000547564.1 NZ_CP040397:c4284279-4283968 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T125744 NZ_CP040397:c4284279-4283968 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT125744 WP_000126294.1 NZ_CP040397:c4283971-4283552 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT125744 NZ_CP040397:c4283971-4283552 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5