Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3518831..3519333 Replicon chromosome
Accession NZ_CP040397
Organism Escherichia coli strain BA22372

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FEV15_RS17215 Protein ID WP_000767829.1
Coordinates 3518831..3519085 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FEV15_RS17220 Protein ID WP_001259255.1
Coordinates 3519082..3519333 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEV15_RS17185 3513846..3514073 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
FEV15_RS17190 3514087..3515145 - 1059 WP_000492346.1 transport protein YeeE -
FEV15_RS17195 3515324..3516682 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FEV15_RS17200 3516672..3516734 - 63 WP_010723108.1 membrane protein YoeI -
FEV15_RS17205 3516949..3517878 - 930 WP_000803351.1 LysR family transcriptional regulator -
FEV15_RS17210 3517924..3518748 - 825 WP_000754758.1 SDR family oxidoreductase -
FEV15_RS17215 3518831..3519085 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FEV15_RS17220 3519082..3519333 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FEV15_RS17225 3519617..3519667 + 51 WP_001364200.1 his operon leader peptide -
FEV15_RS17230 3519813..3520712 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FEV15_RS17235 3520718..3522022 + 1305 WP_001317939.1 histidinol dehydrogenase -
FEV15_RS17240 3522019..3523089 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
FEV15_RS17245 3523089..3524156 + 1068 WP_000080060.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T125742 WP_000767829.1 NZ_CP040397:c3519085-3518831 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T125742 NZ_CP040397:c3519085-3518831 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT125742 WP_001259255.1 NZ_CP040397:c3519333-3519082 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT125742 NZ_CP040397:c3519333-3519082 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References