Detailed information of TA system TA125689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5102604..5103206
• Replicon: chromosome
• Accession: NZ_CP040390
• Organism: Escherichia coli strain A1_136

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FE198_RS25270
• Protein ID: WP_000897305.1
• Coordinates: 5102895..5103206 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FE198_RS25265
• Protein ID: WP_000356395.1
• Coordinates: 5102604..5102894 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE198_RS25225	5098228..5099130	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FE198_RS25230	5099127..5099762	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FE198_RS25235	5099759..5100688	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
FE198_RS25240	5100870..5101112	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
FE198_RS25245	5101331..5101549	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FE198_RS25255	5101968..5102246	-	279	WP_001315112.1	hypothetical protein	-
FE198_RS25260	5102298..5102519	-	222	WP_001550354.1	hypothetical protein	-
FE198_RS25265	5102604..5102894	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
FE198_RS25270	5102895..5103206	-	312	WP_000897305.1	hypothetical protein	Toxin
FE198_RS25275	5103435..5104343	+	909	WP_001550353.1	alpha/beta hydrolase	-
FE198_RS25280	5104511..5106325	-	1815	WP_001550352.1	hypothetical protein	-
FE198_RS25285	5106741..5107682	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FE198_RS25290	5107727..5108164	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T125689 WP_000897305.1 NZ_CP040390:c5103206-5102895 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T125689 NZ_CP040390:c5103206-5102895 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT125689 WP_000356395.1 NZ_CP040390:c5102894-5102604 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT125689 NZ_CP040390:c5102894-5102604 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7