Detailed information of TA system TA125677

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 3962799..3963170
• Replicon: chromosome
• Accession: NZ_CP040390
• Organism: Escherichia coli strain A1_136

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: FE198_RS19590
• Protein ID: WP_001317028.1
• Coordinates: 3962976..3963170 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	3962799..3962977 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE198_RS19565	3958754..3960127	+	1374	WP_001551034.1	ATP-dependent RNA helicase DbpA	-
FE198_RS19570	3960256..3961191	-	936	WP_000662472.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
FE198_RS19575	3961243..3962478	-	1236	WP_001551035.1	site-specific integrase	-
FE198_RS19580	3962480..3962695	-	216	WP_000079604.1	excisionase XisR	-
FE198_RS19585	3962795..3962983	-	189	WP_001551036.1	DUF1187 family protein	-
-	3962799..3962977	+	179	NuclAT_0	-	Antitoxin
-	3962799..3962977	+	179	NuclAT_0	-	Antitoxin
-	3962799..3962977	+	179	NuclAT_0	-	Antitoxin
-	3962799..3962977	+	179	NuclAT_0	-	Antitoxin
FE198_RS19590	3962976..3963170	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
FE198_RS19595	3963227..3964036	-	810	WP_001551037.1	recombination protein RecT	-
FE198_RS19600	3964029..3966680	-	2652	WP_001551038.1	exodeoxyribonuclease VIII	-
FE198_RS19605	3966782..3967057	-	276	WP_001314664.1	hypothetical protein	-
FE198_RS19610	3967132..3967302	-	171	WP_001551041.1	phage protein	-
FE198_RS19615	3967302..3967523	-	222	WP_000560221.1	killing protein KilR	-
FE198_RS19620	3967944..3968096	-	153	WP_001551042.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3950495..4008533	58038

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T125677 WP_001317028.1 NZ_CP040390:c3963170-3962976 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T125677 NZ_CP040390:c3963170-3962976 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT125677 NZ_CP040390:3962799-3962977 [Escherichia coli]
GAGGACTGAAGTCTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGGCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin