Detailed information of TA system TA125674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3141475..3142169
• Replicon: chromosome
• Accession: NZ_CP040390
• Organism: Escherichia coli strain A1_136

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: FE198_RS15565
• Protein ID: WP_001263491.1
• Coordinates: 3141771..3142169 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: FE198_RS15560
• Protein ID: WP_000554755.1
• Coordinates: 3141475..3141768 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE198_RS15535	3137096..3137452	-	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
FE198_RS15540	3137445..3137723	-	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
FE198_RS15545	3137828..3139567	-	1740	WP_001550652.1	flagellar biosynthesis protein FlhA	-
FE198_RS15550	3139512..3140297	+	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
FE198_RS15555	3140368..3141423	+	1056	WP_001550655.1	DNA polymerase IV	-
FE198_RS15560	3141475..3141768	+	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FE198_RS15565	3141771..3142169	+	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FE198_RS15570	3142179..3142631	+	453	WP_019842500.1	GNAT family N-acetyltransferase	-
FE198_RS15575	3142877..3143083	+	207	Protein_2996	RtcB family protein	-
FE198_RS26330	3143291..3143431	+	141	WP_023063669.1	peptide chain release factor	-
FE198_RS15585	3143488..3144945	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
FE198_RS15590	3145206..3145664	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FE198_RS15595	3145756..3147000	+	1245	WP_000189541.1	esterase FrsA	-
-	3146260..3146340	+	81	NuclAT_11	-	-
-	3146260..3146340	+	81	NuclAT_11	-	-
-	3146260..3146340	+	81	NuclAT_11	-	-
-	3146260..3146340	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T125674 WP_001263491.1 NZ_CP040390:3141771-3142169 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T125674 NZ_CP040390:3141771-3142169 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT125674 WP_000554755.1 NZ_CP040390:3141475-3141768 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT125674 NZ_CP040390:3141475-3141768 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5