Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2016897..2017399 Replicon chromosome
Accession NZ_CP040390
Organism Escherichia coli strain A1_136

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FE198_RS09865 Protein ID WP_000767829.1
Coordinates 2017145..2017399 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FE198_RS09860 Protein ID WP_001259255.1
Coordinates 2016897..2017148 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE198_RS09835 2012075..2013142 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FE198_RS09840 2013142..2014212 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
FE198_RS09845 2014209..2015513 - 1305 WP_039267630.1 histidinol dehydrogenase -
FE198_RS09850 2015519..2016418 - 900 WP_000131789.1 ATP phosphoribosyltransferase -
FE198_RS09855 2016564..2016614 - 51 WP_071593552.1 his operon leader peptide -
FE198_RS09860 2016897..2017148 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FE198_RS09865 2017145..2017399 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FE198_RS09870 2017482..2018306 + 825 WP_000754737.1 SDR family oxidoreductase -
FE198_RS09875 2018352..2019281 + 930 WP_000803351.1 LysR family transcriptional regulator -
FE198_RS09880 2019496..2019558 + 63 WP_010723108.1 membrane protein YoeI -
FE198_RS09885 2019548..2020906 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FE198_RS09890 2021085..2022143 + 1059 WP_023568741.1 transport protein YeeE -
FE198_RS09895 2022157..2022384 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T125662 WP_000767829.1 NZ_CP040390:2017145-2017399 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T125662 NZ_CP040390:2017145-2017399 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT125662 WP_001259255.1 NZ_CP040390:2016897-2017148 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT125662 NZ_CP040390:2016897-2017148 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References