Detailed information of TA system TA125661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1214713..1215440
• Replicon: chromosome
• Accession: NZ_CP040390
• Organism: Escherichia coli strain A1_136

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: FE198_RS06015
• Protein ID: WP_000547555.1
• Coordinates: 1214713..1215024 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FE198_RS06020
• Protein ID: WP_000126294.1
• Coordinates: 1215021..1215440 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE198_RS05990	1210648..1212357	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
FE198_RS05995	1212367..1212909	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
FE198_RS06000	1212909..1213676	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
FE198_RS06005	1213673..1214083	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
FE198_RS06010	1214076..1214546	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
FE198_RS06015	1214713..1215024	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
FE198_RS06020	1215021..1215440	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
FE198_RS06025	1215519..1216943	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
FE198_RS06030	1216952..1218409	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FE198_RS06035	1218669..1219679	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
FE198_RS06040	1219828..1220355	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T125661 WP_000547555.1 NZ_CP040390:1214713-1215024 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T125661 NZ_CP040390:1214713-1215024 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT125661 WP_000126294.1 NZ_CP040390:1215021-1215440 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT125661 NZ_CP040390:1215021-1215440 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2