Detailed information of TA system TA125657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 670890..671689
• Replicon: chromosome
• Accession: NZ_CP040390
• Organism: Escherichia coli strain A1_136

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F0JQM9
• Locus tag: FE198_RS03315
• Protein ID: WP_000347270.1
• Coordinates: 670890..671354 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A0V9H1L4
• Locus tag: FE198_RS03320
• Protein ID: WP_001551693.1
• Coordinates: 671354..671689 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE198_RS03285	665891..666325	-	435	WP_000948837.1	PTS sugar transporter subunit IIA	-
FE198_RS03290	666343..667221	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
FE198_RS03295	667211..667990	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
FE198_RS03300	668001..668474	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
FE198_RS03305	668497..669777	-	1281	WP_001551694.1	tagatose-bisphosphate aldolase subunit KbaZ	-
FE198_RS03310	670026..670835	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
FE198_RS03315	670890..671354	-	465	WP_000347270.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
FE198_RS03320	671354..671689	-	336	WP_001551693.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
FE198_RS03325	671838..673409	-	1572	WP_001273738.1	galactarate dehydratase	-
FE198_RS03330	673784..675118	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
FE198_RS03335	675134..675904	+	771	WP_001551692.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T125657 WP_000347270.1 NZ_CP040390:c671354-670890 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T125657 NZ_CP040390:c671354-670890 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12347.87 Da
• Isoelectric Point: 4.6890

>AT125657 WP_001551693.1 NZ_CP040390:c671689-671354 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT125657 NZ_CP040390:c671689-671354 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0V9NQ90

Antitoxin

• AlphaFold DB: A0A0V9H1L4