Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 245258..245793 Replicon plasmid unnamed1
Accession NZ_CP040369
Organism Enterococcus faecium strain VB3240

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag FEF08_RS15725 Protein ID WP_002314366.1
Coordinates 245258..245530 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag FEF08_RS15730 Protein ID WP_002297422.1
Coordinates 245530..245793 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEF08_RS15680 240406..240879 - 474 Protein_268 TlpA family protein disulfide reductase -
FEF08_RS15995 240969..241511 - 543 WP_002298088.1 hypothetical protein -
FEF08_RS15695 241786..242429 + 644 Protein_270 transposase -
FEF08_RS15700 242505..243185 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
FEF08_RS15705 243244..243432 - 189 Protein_272 hypothetical protein -
FEF08_RS15710 243433..244290 - 858 WP_002292680.1 AAA family ATPase -
FEF08_RS15715 244575..244862 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
FEF08_RS15720 244852..245181 - 330 WP_002292678.1 AbrB family transcriptional regulator -
FEF08_RS15725 245258..245530 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
FEF08_RS15730 245530..245793 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FEF08_RS15735 246027..246935 + 909 Protein_278 IS982-like element ISEfm1 family transposase -
FEF08_RS15740 246938..248113 - 1176 WP_002336205.1 MFS transporter -
FEF08_RS15745 248116..250365 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(2'')-Ia / aac(6')-aph(2'') - 1..258646 258646
- inside IScluster/Tn - - 233696..256394 22698


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T125654 WP_002314366.1 NZ_CP040369:c245530-245258 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T125654 NZ_CP040369:c245530-245258 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT125654 WP_002297422.1 NZ_CP040369:c245793-245530 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT125654 NZ_CP040369:c245793-245530 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References