Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 359109..359706 Replicon chromosome
Accession NZ_CP040363
Organism Klebsiella pneumoniae strain R50

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag FEM43_RS02200 Protein ID WP_004142563.1
Coordinates 359389..359706 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag FEM43_RS02195 Protein ID WP_004142561.1
Coordinates 359109..359396 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEM43_RS02165 355190..355438 + 249 WP_002893037.1 DUF1158 domain-containing protein -
FEM43_RS02170 355456..355797 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
FEM43_RS02175 355828..356943 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
FEM43_RS02180 357122..357706 + 585 WP_002893026.1 TetR/AcrR family transcriptional regulator -
FEM43_RS02185 357703..358071 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
FEM43_RS02190 358191..358844 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
FEM43_RS02195 359109..359396 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
FEM43_RS02200 359389..359706 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FEM43_RS02205 359891..360934 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
FEM43_RS02215 361601..362467 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
FEM43_RS02220 362576..364003 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T125635 WP_004142563.1 NZ_CP040363:c359706-359389 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T125635 NZ_CP040363:c359706-359389 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT125635 WP_004142561.1 NZ_CP040363:c359396-359109 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT125635 NZ_CP040363:c359396-359109 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References